Frequency of PGP and MRPA efflux pump genes in drug resistance in clinical isolates of Leishmania tropica and L. major

Parishan, Maede; Nateghi, Mahmoud

Volume 9, Issue 4 (3-2023) nbr 2023, 9(4): 257-266 | Back to browse issues page

‎ 20.1001.1.24236330.1401.9.4.2.4

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Parishan M, Nateghi M. Frequency of PGP and MRPA efflux pump genes in drug resistance in clinical isolates of Leishmania tropica and L. major . nbr 2023; 9 (4) :257-266
URL: http://nbr.khu.ac.ir/article-1-3486-en.html

Frequency of PGP and MRPA efflux pump genes in drug resistance in clinical isolates of Leishmania tropica and L. major

Maede Parishan ^*

, Mahmoud Nateghi

Microbial Biotechnology, Payame Noor University of East Tehran, Tehran, Iran , maede_parishan@yahoo.com

Abstract: (1809 Views)

This study aimed to identify PGP and MRPA genes in clinical isolates of Leishmania. The genes of pgpa (MRPA) and mdr1 (PGP) are involved in the drug resistance, their products act as dependent transporters of ATP (ABC Transporter) in the reflux of drugs from the cytosol to the outer space of the cell. Hence, 40 volunteers with leishmaniasis were randomly selected. Firstly, Amastigotes were examined under a light microscope, then inoculated into NNN-specific biphasic culture medium. Deoxy ribonucleic acids were extracted by phenol-chloroform method and were determined by ITS-specific primers. Then the frequency of two pumps involved in "drug resistance" was investigated by PCR. In this study, the mdr1 gene, which had previously been shown to be present in the in vitro resistant strains, was shown to have a higher frequency of pgpas, which could be due to the presence of MDR. It transports the drug from the inner layers of the lipid bilayer membrane to the outer layers, reducing the concentration of the drug inside the cell and causing drug resistance, while the MRPA pump is in the membrane of the cell organelles.

Keywords: ABC transporter, cutaneous leishmaniasis, glucantim, mdr1 gene, pgpa gene

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