Volume 5, Issue 4 (3-2019)                   nbr 2019, 5(4): 466-478 | Back to browse issues page

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Mohammadi Bazargani M. Comparative analyses of phytochemical compounds of Epilobium minutiflorum (Onagraceae) at different altitudest altitudes. nbr 2019; 5 (4) :466-478
URL: http://nbr.khu.ac.ir/article-1-3157-en.html
Comparative analyses of phytochemical compounds of Epilobium minutiflorum (Onagraceae) at different altitudest altitudes
Mitra Mohammadi Bazargani *
Iranian Research Organization for Science and Technology , mitra_cb@yahoo.com
Abstract:   (5228 Views)
Epilobium minutiflorum belongs to the genus Epilobium with important medicinal properties. In this study, the natural variation of phytochemical compounds of E. minutiflorum at different altitudes has been investigated. Aerial parts of plants were collected at flowering stage from natural habitats at different altitudes of 2387, 2569 and 2813 masl. The plant extracts were obtained with 80% methanol and several phytochemical properties were measured. The properties included the total flavonoid content (TFL), total phenol (TPH), antioxidant capacity (AOX) and anthocyanin content (ACY) measured by the AlCl3, the Folin-Ciocalteu, the Ferric reducing antioxidant power (FRAP) and the differential PH methods, respectively. In addition, phytochemical components were identified by the GC/MS method. The results of photometric analysis indicated that the population of Shemshak, located at the highest altitude showed the highest content of TFL, TPH and AOX with 30.39 mg/gr DW, 938.91 mg/gr DW and 77.77 mM/gr DW, respectively. The TFL, TPH and AOX values showed a positive correlation with altitude. The results of GC/MS analysis revealed the presence of 41 compounds in E. minutiflorum, which were separated into flavonoids, phenolic acid and its derivatives, steroids and terpenes groups. Shemshak population showed the highest value in all identified compounds. A positive correlation was also observed between altitude and 30 compounds identified by the GC/MS method including mainly flavonoids and phenolics. The results of this study indicated that environmental factors at higher altitudes may influence the elevation of the flavenols and phenol content in E. minutiflorum. The identification of these influential factors on phytochemical content at higher altitudes requires further studies.

 
 
Keywords: antioxidant, flavonoeid, GC/MS, phenolic acid, phytochemical variation
Full-Text [PDF 1191 kb]   (1522 Downloads)    
Type of Study: Original Article | Subject: Plant Biology
Received: 2018/07/23 | Revised: 2019/04/6 | Accepted: 2018/10/3 | Published: 2019/03/18 | ePublished: 2019/03/18
References
1. Araújo, L.U., Grabe-Guimarães, A., Mosqueira, V.C.F., Carneiro, C.M., and Silva-Barcellos, N.M. 2010. Profile of wound healing process induced by allantoin. – Acta Cir. Bras. 25: 460-461. [DOI:10.1590/S0102-86502010000500014]
2. Arredondo, M., Blasina, F., Echeverry, C., Morquio, A., Ferreira, M., Abin-Carriquiry, J., Lafon, L., and Dajas, F. 2004. Cytoprotection by Achyrocline satureioides (Lam) DC and some of its main flavonoids against oxidative stress. – J. Ethnopharmacol. 91: 13-20. [DOI:10.1016/j.jep.2003.11.012]
3. Barrett, S.A., and Gifford, E.W. 1933. Indian life of the yosemite region: Miwok material culture. Bull. Public Mus. – Milwaukee. 2: 174. ب
4. Bazylko, A., Kiss, A., and Kowalski, J. 2007. Densitometric determination of flavonoids in methanolic and aqueous extracts of Epilobii angustifolii herba by use of HPTLC. J. Planar. Chromatogr. – Mod. TLC. 20: 53-56.
5. Bocek, B.R. 1984. Ethnobotany of Costanoan Indians,California,based on collections by John P. Harrington. – Econ. Bot. 38: 240-255. [DOI:10.1007/BF02858839]
6. Ducrey, B., Wolfender, J., Marston, A., and Hostettmann, K. 1995. Analysis of flavonol glycosides of thirteen Epilobium species (Onagraceae) by LC-UV and thermospray LC-MS. – Phytochemistry. 38: 129-137. [DOI:10.1016/0031-9422(94)00629-8]
7. Glen, A.T., Lawrie, W., McLean, J., and Younes, M.E.-G. 1967. Triterpenoid constituents of rose-bay willow-herb. – J. Chem. Soc. C. 510-515. [DOI:10.1039/j39670000510]
8. Granica, S., Piwowarski, J.P., Czerwińska, M.E., and Kiss, A.K. 2014. Phytochemistry, pharmacology and traditional uses of different Epilobium species (Onagraceae): a review. – J. Ethnopharmacol. 156: 316-346. [DOI:10.1016/j.jep.2014.08.036]
9. Hevesi Tóth, B. 2009. Phytochemical and in vitro biological evaluation of potentially active compounds in Epilobium species. Thesis of doctoral (Ph. D.) dissertation Budapest: Semmelweis University.
10. Hevesi Tóth, B., Balázs, A., Vukics, V., Szőke, É., and Kéry, Á. 2006. Identification of Epilobium species and willow-herbs (Onagraceae) by HPLC analysis of flavonoids as chemotaxonomic markers. – Chroma. 63: 119-123. [DOI:10.1365/s10337-006-0740-y]
11. Hevesi Tóth, B., Blazics, B., and Kéry, Á. 2009. Polyphenol composition and antioxidant capacity of Epilobium species. J. Pharm. – Biomed. Anal. 49: 26-31. [DOI:10.1016/j.jpba.2008.09.047]
12. Hiermann, A. 1995. Phytochemical characterization of Epilobium angustifolium L. and its differentiation to other Epilobium species by TLC and HPLC. – Sci. Pharm. 63: 135-135.
13. Hiermann, A., and Mayr, K. 1985. The investigation of active compounds from Epilobium species. The occurrence of sitosterol derivatives in Epilobium angustifolium L. and Epilobium parviflorum Schreb. – Sci. Pharm. 53: 39-44.
14. Ismail, N.Z., Arsad, H., Samian, M.R., and Hamdan, M.R. 2017. Determination of phenolic and flavonoid contents, antioxidant activities and GC-MS analysis of Clinacanthus nutans (Acanthaceae) in different locations. – Agrivita 39: 335–344. [DOI:10.17503/agrivita.v39i3.1076]
15. Katalinic, V., Milos, M., Kulisic, T., and Jukic, M. 2006. Screening of 70 medicinal plant extracts for antioxidant capacity and total phenols. – Food Chem. 94: 550-557. [DOI:10.1016/j.foodchem.2004.12.004]
16. Miguel, M.G. 2011. Anthocyanins: antioxidant and/or anti-inflammatory activities. – J. Appl. Pharm. Sci. 1: 7-15.
17. Monschein, M., Jaindl, K., Buzimkić, S., and Bucar, F. 2015. Content of phenolic compounds in wild populations of Epilobium angustifolium growing at different altitudes. – Pharm. Biol. 53: 1576-1582. [DOI:10.3109/13880209.2014.993039]
18. Rohloff, J., Uleberg, E., Nes, A., Krogstad, T., Nestby, R., and Martinussen, I. 2015. Nutritional composition of bilberries (Vaccinium myrtillus L.) from forest fields in Norway–Effects of geographic origin, climate, fertilization and soil properties. – J. Appl. Bot. Food Qual. 88 274-287.
19. Shikov, A.N., Poltanov, E.A., Dorman, H.D., Makarov, V.G., Tikhonov, V.P., and Hiltunen, R. 2006. Chemical composition and in vitro antioxidant evaluation of commercial water-soluble willow herb (Epilobium angustifolium L.) extracts. – J. Agric. Food Chem. 54: 3617-3624. [DOI:10.1021/jf052606i]
20. Shikov, A.N., Pozharitskaya, O.N., Ivanova, S.A., Makarov, V.G., Tikhonov, V.P., and Galambosi, B. 2010. Improved and validated HPTLC method for quantification of oenothein B and its use for analysis of Epilobium angustifolium L. – J. Planar. Chromatogr. Mod. TLC. 23: 70-74. [DOI:10.1556/JPC.23.2010.1.12]
21. Slacanin, I., Marston, A., Hostettmann, K., Delabays, N., and Darbellay, C. 1991. Isolation and determination of flavonol glycosides from Epilobium species. – J. Chromatogr. A. 557: 391-398. [DOI:10.1016/S0021-9673(01)87147-8]
22. Smith, H.H. 1923. Ethnobotany of the Menomini Indians. Bull. Public Mus. – Milwaukee. 19234: 40.
23. Thornfeldt, C. 2005. Cosmeceuticals Containing Herbs: Fact, Fiction, and Future. – Dermatol. Surg. 31: 873-881. [DOI:10.1111/j.1524-4725.2005.31734]
24. Tyler, V. 1986. Some recent advances in herbal medicine. – Pharm Int. 7: 203-207.
25. Veraldi, S., De, P.M., Schianchi, R., and Lunardon, L. 2009. Treatment of pruritus in mild-to-moderate atopic dermatitis with a topical non-steroidal agent. Journal of drugs in dermatology: – JDD. 8: 537-539.
26. Wojdyło, A., Oszmiański, J., and Czemerys, R. 2007. Antioxidant activity and phenolic compounds in 32 selected herbs. – Food Chem. 105: 940-949. [DOI:10.1016/j.foodchem.2007.04.038]
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