Volume 6, Issue 2 (8-2019)
nbr 2019, 6(2): 176-183 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Heidargholinejad F, Moradi H, Karimi M, Akbarpour V. Callus induction in Portulaca oleracea L. by different hormone concentrations and explant types. nbr 2019; 6 (2) :176-183
URL: http://nbr.khu.ac.ir/article-1-2972-en.html
URL: http://nbr.khu.ac.ir/article-1-2972-en.html
Sari Agricultural Sciences Natural Resources University , moradiho@yahoo.com
Abstract: (4947 Views)
Purslane (Portulaca oleracea L.) contains valuable secondary metabolites such as Dopamin, Noradrenaline and Omega-3. This plant is used in various medicinal, food and hygienic industries as well as the treatment of different diseases such as diabetes, heart disease and pain relief. Callus induced from medicinal plants are used to increase the production of secondary metabolities in cell suspension culture and gene transfer. The purpose of this experiment was the study of different concentrations of BAP and 2,4-D of two explants from leaf and shoot tips to produce callus. Leaf and shoot tip explants were used in MS with different concentrations of BAP at three levels (0, 1 and 2 mg/L) with 2,4-D at three levels (0, 0.5 and 1.5 mg/L). Results showed that interactions between hormones and explants were significant in the percentage of callus induction, fresh weight and callus diameter at 1% level. The best result which was the leaf explant with 100% callus induction, 121 mg fresh weight and 5.106 mm callus diameter was obtained by the combination of BAP 2 mg/L and 2,4-D 0.5 mg/L. Shoot tip explants with 75% callus induction, 106 mg fresh weight and 3.03 mm diameter was obtained by the application of 1 mg/L BAP and 0.5 mg/L 2,4-D.
Type of Study: Original Article |
Subject:
Plant Biology
Received: 2017/09/29 | Revised: 2019/10/5 | Accepted: 2018/04/24 | Published: 2019/07/30 | ePublished: 2019/07/30
Received: 2017/09/29 | Revised: 2019/10/5 | Accepted: 2018/04/24 | Published: 2019/07/30 | ePublished: 2019/07/30
References
1. Abbasi, B., Saxena, P.K., Murch, S.J. and Liu, C.Z. 2007. Echinacea botechnology: challenges and opportunities. - In vitro Cel. Dev. Bio. 43: 481-492. [DOI:10.1007/s11627-007-9057-2]
2. Abdelmageed, A.H.A., Faridah, Q.Z., Norshuhada, K. and Julia, A.A. 2012. Call induction and plant regeneration of Michelia champaca L. (Magnoliaceae). - J. Med. Pla. Re. 6: 3338-3344. [DOI:10.5897/JMPR12.196]
3. Abubakar, D., Bakrudeen, A.A. and Rosna, M.T. 2014. In vitro callus induction and plant regeneration of Celosia argenta L. an important medicinal plant. - Bra. Arc. Bio. Tech. Int. J. 57: 860-866. [DOI:10.1590/S1516-8913201402611]
4. Ahmad, N., Fazal, H. and Zamir, R. 2011. Callogenesis and shoot organogenesis from flowers of Stevia rebaudiana Bertoni Bert. - Su. Tec. 13: 174-177. [DOI:10.1007/s12355-011-0083-3]
5. Ahmadi, G., Mohammadi, R., Garousi, KH. and Hosseini, R. 2012. Optimization callus and cell suspension (Catharanthus roseus L.). - J. Agr. Bio. 4: 157-163.
6. Alagumanian, S., Perumal, V.s., Rameshkannar, R. and Rao, M.V. 2004. Plant regeneration from leaf and stem explants of Solanum trilobatum L. - Cur. Sci. 86: 1478-1480.
7. Arafeh, R.M., Shibli, R.A., Al-Mahmoud, M. and Shatanwi, M.A. 2006. Callusing cell suspension culture and secondary metabolites production in Persian oregano (Origanum vulgar L.) and Arabian oregano (O. syriacum L.). - J. Agr. Sci. 2: 274-281.
8. Arda, A., Ozen, F. and Kiran, R. 2012. Development of an efficient callus production protocol for Amsonia orientakis: A critically endangered medicinal plant. - E. A. J. Bio. 6: 105-112. [DOI:10.5053/ejobios.2012.6.0.13]
9. Bakhtiar, Z., Mirjalilian, M.H. and Sonboli, A. 2016. In vitro callus induction and micropropagation of Thymus persicus L. (Lamiaceae), an endangered medicinal plant. - Cr. Br. Ap. Bio.16: 48-54. [DOI:10.1590/1984-70332016v16n1a8]
10. Borijian, L. and Arak, H. 2013. A study on the effect of different concentration of plant hormones (BAP, NAA, 2,4-D, Kinetin) on callus induction in Brassica napus. - J. Ap. Ba. Sci. 5: 519-521.
11. Chen, J., Shi, Y.p. and Liu, J.Y. 2003. Determination of noradrenaline and dopamin in chines herbal extract from Portulaca oleracea L. by high-performance liquid chromatography. - J. Chr. A. 1003: 127-132. [DOI:10.1016/S0021-9673(03)00786-6]
12. Dighe, V., Dhotre, O., Gaurang, P. and Gursale, A. 2008. Quantification of dopamine in Portulaca oleracea L. by high-performanc thin layer chromatography. - J. Pla. Mat. 21: 183-186. [DOI:10.1556/JPC.21.2008.3.5]
13. Jala, Anchalee. 2014. Role of 2,4-D on callus induction and shoot formation of increase number of shoot in Miniature Rose In vitro. - Am. Tra. Eng. Ap. Sci. 3: 207-212.
14. Kouhi, L., Zare, N., Asghari, Z. and Shiekhzadehm-ossadegh, P. 2014. The role of plant growth regulators and different explants on reply tissue culture and cell suspention (Matricaria chomomilla L.). - Eco. Cr. J. 8: 203-214.
15. Magyar-Taboti, k., Dobranszki, J., Teixeira, D.A. and Silva, J.A. 2010. The role of cytokinins in shoot orga-nogenesis in apple. - Plant Cell Tis. Org. Cul. 101: 251-267. [DOI:10.1007/s11240-010-9696-6]
16. Mahmood, I., Razzaq, A., Khan, Z., Hafez, I.A. and Kaleem, S. 2012. Valuation of tissue culture responses of promising Wheat (Triticum aestivum L.) cultivars and development of efficient regen-eration system. - Pa. J. Bot. 44: 277-284.
17. Maziard, M., Khan, T. and Mohammad, F. 2011. Role of secondary metabolites in defense mechanism of plants. - Bio. Med. 3: 232-249.
18. Pirian, K., and Piri, Kh. 2014. Callus induction in hairy roots of Portulaca oleracea L. - Ir. J. Med. Aro. Plants. 30: 231-238.
19. Safdari, Y. and Kazemitabar, S.K. 2009. Plant tissue culture study on two different races of purslane (Portulaca oleracea L). - Af. J. Bio. 8: 5906-5912. [DOI:10.5897/AJB09.816]
20. Safdari, Y. and Kazemitabar, S.K. 2010. Direct shoot regeneration, callus induction and plant regeneration from callus tissue in Mose Rose (Portulaca grandiflora Hook.). - Plant Oman J. 3: 47-51.
21. Saikia, M., Shrivastava, K. and Sigh, S. 2013. Effect of culture media and growth hormone on callus induction in Aquilaria malaccensis Lam., a medicinally and commercially important tree species north east Indian. - As. J. Bio. Sci. 6: 96-105. [DOI:10.3923/ajbs.2013.96.105]
22. Saleh, R., Ghorbani, M., and Khalife, S. 2016. Effect of various concentrations of different growth regulating hormones on callus weight and the amount of thymol of Thymus daenensis Celak. - Plant Phy. 7: 1919-1923.
23. Sharafi, A., Hashemi, S.H., and Jourabchi, E. 2008. Improvement on the situation of regeneration of medicinal plant Artemisia annua L. - J. Enviro. Re. 21: 565-573.
24. Soltanipol, M., Mohammadi, A., Rahnama, H. and Abbaszadeh, B. 2011. Callus induction in (Melissa officinalis L.). - J. Agri. Crop Improv. 7: 45-54.
25. Teshome, S. and Feyssia, T. 2015. In vitro callus inductin and shoot regeneration from leaf explant s of Glinus lotoides L. L. an important medicinal plant. - Am. J. Plant Sci. 6: 1329-1340. [DOI:10.4236/ajps.2015.69132]
26. Urrea, A.I., Castrillon, P.A. and Monsalve, Z. 2009. In vitro propagation and tisular differentiation in Lippia dulcis Trevir.- Actualidades Biol. 31: 21-29.
27. Wani, M., Pande, S. and More, N. 2010. Callus induction studies in Tridax procumbens L. - Inter. J. Biotechnol. Appli. 2: 11-14. [DOI:10.9735/0975-2943.2.1.11-14]
28. Yang, X., Zhang, X., Yuan, D., Jin, F., Zhang, Y. and Xu, J. 2012. Transcript profiling reveals complex auxin signaling pathway and transcription regulation involved in dedifferentiation and redifferentiation during somatic embryogenesis in cotton. - BMC Plant Bio. 12: 102-110. [DOI:10.1186/1471-2229-12-110]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
© 2024 CC BY-NC 4.0 | Nova Biologica Reperta
Designed & Developed by : Yektaweb