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Sex steroid hormones play important roles in many physiological processes, particularly in the reproduction of teleostei. Therefore, study of steroid hormones in fish is one of the important indicators of reproductive biology. In the present research, serum steroid hormones such as testosterone (T), 17β-estradiol (E2) and progesterone (P) in Esox lucius brood stocks, during one year, from autumn 2010 to summer 2011, in five periods (autumn, first half of winter, second half of winter, spring and summer) with three longitudinal classes: 300-360, 360-420 and 420-480 millimeters were studied. The results showed significant difference between levels of steroid hormones in three longitudinal classes and showed an increasing trend by increased length (P<0.05). In addition, there was significant different between levels of gonadal steroid hormones and different seasons (P<0.05). The highest level of 17-β estradiol was observed in autumn season. In the case of testosterone and progesterone hormones, higher levels were recorded in first and second half of winter, respectively.