Showing 3 results for Seedling
Tahereh Naeemi, , Baratali Fakheri,
Volume 6, Issue 2 (8-2019)
Abstract
Plant growth is greatly influenced by environmental stresses including water deficit, salinity and extreme temperatures. Therefore, the identification of genes, especially regulatory ones whose expression enables plants to adapt to or to tolerate these abiotic stresses, is very essential. MYB proteins, a superfamily of transcription factors, play regulatory roles in developmental processes and in defense responses in plants. Members are characterized by a structurally conserved DNA-binding domain, i.e., the MYB domain. Thus, a factorial experiment in a randomized complete block design with three replications was carried out to investigate the effect of different levels of drought stress on the relative expression of TaMYB73 transcription factor gene using Real Time PCR method at Biotechnology Research Institute of Zabol University. The experimental treatments included durum wheat genotypes (Shabrang, Behrang, Karkheh, Aria and Dena) and drought levels (5, 10, 15, 20 and 25% of field capacity). Seedlings of genotypes
were cultured in pots and drought stress was exerted after 45 days (four to five leaves). Data analysis was performed using Ratio =2 -ΔΔCT and SAS software version 9.1. The results of the 2-way ANOVA for the effect of genotype, drought stress and cross-effects of drought stress on the relative expression of TaMYB73 gene and the amount of osmotic regulators (Proline and Carbohydrate) at different stress levels (20, 15, and 5% of field capacity) were significant in comparison with the normal condition (25% of field capacity) at 1% probability level. With the increase of drought stress level from 5 to 20% of crop capacity, relative to the normal level (25% crop capacity), the relative expression of TaMYB73 gene and osmotic regulators of proline and carbohydrates increased in Beharang, Karkheh and Dena genotypes. Therefore, Karkheh and Dena genotypes showed greater resistance to drought stress among the 5 genotypes studied.
Fatemeh Nejadhabibvash, Mohammad Bagher Rezaee,
Volume 7, Issue 4 (12-2020)
Abstract
Beta vulgaris belongs to the family Amaranthaceae and was found to have halophytic ancestors. The objective of this study was to investigate the effect of sodium chloride on seed germination, therefore the early stages of seedling growth of Beta vulgaris grown under different salinity levels (0, 100, 150 and 200 mM of NaCl) were studied. The experiment was laid out in a completely randomized design (CRD) with four replications. It was revealed that as concentration of NaCl increases, the rate and percentage of germination, length and fresh weight of radicle & plumule and seedling vigour index decrease. In conclusion, our results indicated that salt stress decreased Beta vulgaris seed germination and early seedling growth while induced changes in the anatomical characteristics such as increased level of cutin synthesis on epidermal leaves cells and altered the thickness of vascular system, epidermis and parenchyma in leaves, roots and petioles.
Esmaeil Gholinezhad, Saiedeh Salavati, , Bakhtiar Lalehgani,
Volume 13, Issue 1 (4-2026)
Abstract
Objective: This research was conducted to investigate the effects of sowing date and different seed priming treatments on morpho-physiological traits and grain yield of Simin wheat cultivar under late spring cold stress conditions in West Azerbaijan province.
Methods: The experiment was carried out as a factorial arrangement in a randomized complete block design with three replications during the 2024-2025 growing season. The first factor included two sowing dates (November 21 and December 22) and the second factor consisted of seed priming treatments.
Results: The results indicated that the first sowing date showed significant superiority in all traits and led to a 38% increase in grain yield compared to the second sowing date. Among priming treatments, melatonin was identified as the most effective treatment with 37% and 42% increase in grain yield in the first and second sowing dates, respectively. In contrast, salicylic acid treatment in the second sowing date caused 92% reduction in grain yield ratio and 75% reduction in biomass production efficiency.
Conclusion: Optimal sowing date along with the application of priming treatments such as melatonin and gibberellic acid can be recommended as an effective strategy for improving grain yield and late spring cold stress tolerance in wheat.
