Mohsen Shahriari Moghadam, Behrooz Abtahi, Gholamhossein Ebrahimipour,
Volume 6, Issue 2 (8-2019)
Abstract
Organisms in different environmental conditions express different genes, which result in different protein expressions. These changes result from the adaptation of the organism to environmental conditions such as the presence of toxic substances. This study aimed to investigate the changes in protein expression in Celeribacter persicus SBU1 isolated from Nayband Bay mangrove forests, cultured in the medium containing phenanthrene as the sole source of carbon and energy. For this purpose, C. persicus SBU1 was cultured on mineral salt medium containing phenanthrene and sodium acetate as treatment and control, respectively. After the extraction of total protein, changes in protein expression were evaluated by SDS-PAGE. Proteins were identified by MALDI-TOF-TOF MS. After evaluating changes in protein content, two bands which showed greater variation in comparison with the control treatment (increased protein expression) were detected. The identified proteins included one ligand-gated channel protein and one unknown protein. In general, the results of this study showed significant changes in the protein content of C. persicus SBU1 after using phenanthrene. The up-regulation of ligand-gated channel protein signified the role of this protein in phenanthrene molecules transport in and out of the cells.
Ali Jahangiri Zarkani, Mousa Keshavarz, Adnan Shahdadi,
Volume 10, Issue 3 (12-2023)
Abstract
Barnacles are bottom-dwelling crustaceans that live on a variety of substrates, including rocks, mollusk shells, corals, sponges, mangrove roots and leaves, and the body surface of turtles and whales. The present study deals with the species Microeuraphia permitini on the shores of Bandar Abbas in two stations of mangrove forest and rocky beach in a period of two months, and has investigated the morphometrics of the specimens, while considering the effect of the lunar cycle on reproduction, temperature and wind speed of the environment. Five longitudinal parameters (height, basal length and width, opercular length and width) were recorded. The results showed that 90% and 70% of the samples of both stations had eggs in the second day (new moon) and eighth days of lunar months, respectively, and in the twelfth day of the lunar month (full moon) all samples contained eggs. The results showed that there is a significant difference between the height and base length of the barnacle, but the difference between the opercular length, opercular width and base width is not significant. In addition, in both of mangrove and rocky stations, there was the highest correlation coefficient between opercular length and width (r=0.84 and r=0.78, respectively). The lowest correlation coefficient in mangrove station was between base length and opercular width (r=0.5) and in rocky station between height and base width (r=0.2). The wind pattern was variable during this period and the approximate decrease of the north wind was seen during the sampling period. Air temperature changes were seen between minimum 30 °C and maximum 39 °C. In addition to the tidal cycle, wind and temperature can affect the spawning of the species as an environmental stress factor.
