Showing 3 results for Cell Proliferation
Katayoon Meimandi, Mohammad Mehdi Yaghoobi,
Volume 6, Issue 1 (5-2019)
Abstract
In this study, the cytotoxic effects of aqueous and ethanolic extracts of Sedum album L. on human stomach cancer cell line (AGS) and breast cancer cell line (MCF-7) were evaluated by MTT, BrdU and TUNEL assays. The results demonstrated that both extracts had antiproliferative and apoptotic effects in a dose-dependent manner. The MTT assay data revealed that the AGS cell underwent more cytotoxicity in comparison with the MCF-7 cell. It also revealed that ethanolic extract was more potent than aqueous extract. The BrdU assay results showed that the proliferation of AGS and MCF-7 cells was reduced to 50% and 43%, respectively, at the highest concentration of the aqueous extract. In addition, the ethanolic extract reduced the proliferation of AGS and MCF-7 cells to 75% and 60%, respectively. The AGS and MCF-7 cells underwent 52% and 12% apoptotic death upon treatment by the ethanolic extract as TUNEL assay showed. The aqueous extract induced 28% and 25% apoptosis in the AGS and MCF-7 cells, respectively. Both inhibition of proliferation and induction of apoptosis are desirable strategies for cancer treatment among researchers. Identification of S. album compounds and analyzing their effects in animal model of cancer can help us with understanding its anti-cancer properties.
Negar Khorasani, Javad Baharara, Khadijeh Nejad Shahrokhabadi,
Volume 10, Issue 2 (9-2023)
Abstract
Pancreatic cancer is one of the most deadly and aggressive cancers; Fluorouracil induces apoptosis and cell cycle arrest in cancer cells. In the present study; the effect of Fluorouracil on different stages of the cell cycle and the expression of genes involved in the internal pathway of apoptosis in the AsPC-1 cell line (human pancreatic cancer) were investigated. In order to do so, MTT assay was used to evaluate the cytotoxic effect of Fluorouracil on AsPC-1 cell proliferation; The type of induced cell death and cell cycle changes were investigated by flow cytometry; changes in the expression level of genes (BAX, Bcl-2, APAF-1, Caspase-3, Caspase-9, p53, p21) were examined by Real-time PCR. Quantitative data were analyzed at the significant level of (p<0.05). The MTT assay results showed that Fluorouracil decreased AsPC-1 cell proliferation in a concentration-dependent manner. The results of flow cytometry analysis showed that increased percentage of apoptotic cells in the treated cells; Fluorouracil induces S phase cell cycle arrest in AsPC-1 cells and reduced distribution in the G1 phase. The Real-time PCR results in treated cells showed an increase in the expression of genes in the mitochondrial apoptotic pathway as well as genes effective in regulating the cell cycle. Fluorouracil reduces cell proliferation and induces apoptosis by increasing the expression of genes involved in the Intrinsic apoptotic pathway in AsPC-1 cells; Fluorouracil also caused cell cycle arrest in these cells by regulating the (p53, p21) genes.
, , Seyyedeh Mahdokht Maddah,
Volume 12, Issue 1 (3-2025)
Abstract
Gastric cancer is one of the most common cancers, causing significant mortality worldwide. Nepeta species, known for their distinct aroma and taste, have garnered attention in the food industry. Given their phenolic compounds, this study aimed to evaluate the cytotoxic effects of Nepeta binaludensis and N. glomerulosa extracts on AGS gastric cancer cell lines and assess changes in BAX gene expression. Both species were collected from the Binalud Mountains in Khorasan, and their hydroalcoholic extracts were prepared via maceration. The amounts of caffeic acid and Rosmarinic acid were measured using HPLC. The extracts' cytotoxic effects were evaluated with the MTT assay, and apoptosis induction was assessed via DAPI staining. Changes in BAX gene expression were measured using real-time PCR. The IC50 values after 48 hours were 3.70 mg/mL for N. binaludensis extract and 6.92 mg/mL for N. glomerulosa extract. Apoptosis induction was observed under both treatments, but N. binaludensis extract, which contained higher Rosmarinic acid, significantly increased BAX expression compared to the control group. Thus, these extracts may inhibit gastric cancer cell growth, with N. binaludensis showing greater efficacy through BAX-mediated apoptosis induction compared to N. glomerulosa.
