Pardis Ghorbani Salkuyeh, Mohammad Mehdi Sohani, Amin Abedi,
Volume 5, Issue 2 (9-2018)
Abstract
Strictosidine synthase-like (SSL) is a group of gene families in the Arabidopsis genome, which whose orthologues in other plants are key enzymes in mono-terpenoid indole-alkaloid biosynthesis pathway. The SSL7 is upregulated upon treatments of Arabidopsis plants with signaling molecules such as SA, methyl jasmonate and ethylene. To find the functional role of the gene, a T-DNA-mediated knockout mutant (ssl7) along with the wildt ype were treated with different concentrations of NaCl. The expression level of salt stress genes including P5CS1, NCED3, AAO3 and RD29A at 150 mM NaCl demonstrated that the expression was significantly higher in ssl7 compared with the expression in Col-0. The activities of Catalase (CAT), Ascorbate Peroxidase (APX), Peroxidase (POD) and Superoxide Dismutase (SOD) were measured in different concentrations of NaCl. The results suggested that the enzymes activities were significantly higher in ssl7 compared with wild-type Col-0. In total, the results suggest that SSL7 might have a salicylic acid-dependent negative regulatory role in plant resistance to salt stress.
Rezvan Mohammadi, Parto Roshandel, ,
Volume 6, Issue 1 (5-2019)
Abstract
In order to study the effects of magnetopriming on the physiological and phytochemical characteristics of Hyssopus officinalis plants, this research was conducted as a factorial experiment in a completely randomized design. Results showed that magnetopriming (particularly at 200mT/5 min) increased the level of shoot dry weight (82.6 percent), root dry weight (86.5 percent), total chlorophyll (32.8 percent), carotenoids concentration (32.4 percent) and polyphenols content (2 folds) in 60-day-old Hyssopus officinalis. Also, electrolyte leakage and lipid peroxidation decreased by 27.6 and 45 percent, respectively. In addition, reducing power, DPPH and superoxide anion scavenging activities significantly augmented. However, higher activities of superoxide dismutase (76 percent), catalase (4.2 folds), ascorbate peroxidase (2.4 folds) and guaiacol peroxidase (48 percent) were found at 90 mT. Results suggested that the application of magnetopriming promoted growth in H. officinalis through augmentation of cellular membrane integrity as well as biomass and photosynthetic pigments content. Furthermore, it was found to enhance the antioxidative system. Magnetopriming might apparently improve the medicinal properties via increasing the level of polyphenols and antioxidant capacity in H. officinalis.
Ebrahim Shirinpour, Maryam Nasra Esfahani, Davood Bakhshi,
Volume 7, Issue 2 (7-2020)
Abstract
Phosphorus
is an essential nutrient for plant growth and productivity. Since agricultural soils in Iran are predominantly calcareous with very low available Pi content, Pi deficiency has been considered to be a major nutritional constraint for crop production, thus, the application of Pi-fertilizers is essential for satisfactory crop production. The application of Pi-fertilizers contaminates soil and water resources. Therefore, the application of Pi-fertilizers should be reduced through some efficient strategies. The identification of genotypes more tolerant to Pi deficiency is an important low-cost strategy to promote sustainable agriculture in low fertility soils. In this study, the morphological and biochemical responses of five cultivars of common bean (Talash, Mahali Khomein, Sadri, Kosha and Line Ks21191) were evaluated under Pi sufficiency and Pi deficiency. Under Pi-deficient conditions, fresh and dry weights and shoot length were lower while root length was higher in comparison with Pi-sufficient conditions. Under Pi-deficient conditions, the highest and lowest levels of total P were observed in Mahali Khomein and Talash, respectively. The activities of superoxide dismutase and peroxidase in root and catalase in leave showed remarkable increase under Pi-deficient conditions. In conclusion, Mahali Khomein and Talash were the most and the least Pi-deficient tolerant cultivars, respectively.