Showing 2 results for Agrobacterium
Reza Norouzi1, Mesbah Babalar, Masoud Mirmasoumi3 ,
Volume 4, Issue 2 (9-2017)
Abstract
Hairy root induction in plants is the result of the insertion of T-DNA from Agrobacterium rhizogenes into the plant genome. The present study was conducted to investigate the effect of bacterium strain and plant species type on hairy root induction in two endemic (Salvia eremophila and S. reuterana) and five non-endemic (S. macrosiphon, S. multicaulis, S. nemorosa, S. verticellata and S. virigata) Salvia by four bacteria strains including 1724, 2659, ATCC-15834 and A4. Petiole and stem explants were not capable of inducing hairy roots, while almost all leaf segments produced it. Confirmatory studies were carried out by direct detection of inserted rol C by the PCR. The results showed that different Agrobacterium rhizogenes strain and Salvia spices had significant effect on hairy roots number and frequency. The infection of S. macrosiphon via A. rhizogenes strain ATCC15834 showed the highest number of infe-cted roots per explant (5.12 hairy roots) and root frequency (82%). The highest number of hairy root per explant in S. eremophila (3.32 hairy roots) and S. reuterana (3.92 hairy roots) were achieved by inoculation with strain A4. Strain 2659 produced the highest hairy roots number in S. nemorosa (2.6 hairy roots), S. multicaulis (4.36 hairy roots) and S. verticillata (5 hairy roots). Also hairy roots formation occurred at the highest number in S. virigata (3 hairy roots) with infection by strain ATCC15834.
Elham Mohajel Kazemi, Maghsoud Pazhohandeh, Parisa Jonoubi, Mina Kazemian,
Volume 7, Issue 1 (4-2020)
Abstract
One of the main strategies to improve plant tolerance is the expression of stress-induced genes, which play a significant role in the ionic balance of plants. SOS3 is one of the important components of SOS-regulated ionic homeostasis pathway. Therefore, the expression of this gene could be an important step towards producing salt-resistant plants. In this work, we have transformed tomato (Solanum lycopersicum) by Agrobacterium (GV3101 and LBA4404) containing plasmids with SOS3 genes. The maximum regeneration rate was determined in cotyledons of CH genotype. The simultaneous use of cotyledons and hypocotyls in the culture medium had the best outcome. In addition, the best time was found to be one day after inoculation. Also, the best transgenic variety was detected for Agrobacterium GV3101, which can be attributed to the interaction between the genus Agrobacterium and the tomato variety. Transgenic plants were transferred to a culture medium containing sequestrene, which caused the acceleration of the seedling growth in particular. The presence of the SOS3 in the transgenic plants was verified by PCR and RT-PCR methods.
