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Evaluation of genetic diversity and classification of watermelon (Citrullus lanatus) ecotypes using SRAP molecular markers
Maryam Abdoli Nasab, Mehdi Rahimi,
Volume 4, Issue 3 (12-2017)
Abstract
Thirty eight ecotypes of watermelon were collected from different parts of Iran. After the preparation of the field, these eotypes were cultivated in a completely randomized block design with three replications. In order to invest-igate genetic diversity, genomic DNA samples were extracted from leaves and Polymerase chain reactions were optimized using 14 SRAP primer pairs. One hundred thirty six polymorphic bands were detected, of which the EM10-Me4 was the most abundant primer pair with 19 bands and EM16-Me4 and EM16-Me14 were the least primer pairs with 7 bands. PIC index varied from 0.20 to 0.32 and genetic diversity was 0.17 to 0.28 on the basis of Nei index. Fisher's Linear Detection Analysis showed that the UPGMA method and the grouping accuracy of about 90% are more appropriate than other cluster analysis methods. Cluster analysis, using Jakard method, was performed and the ecotypes studied were classified into five distinct groups. Based on the PCA, the first and second components included 92.5% of the variation, which represents the proper distribution of the markers on the whole genome.
Induced genetic and morphological changes in Catharanthus roseus L. by cold atmospheric plasma
Zahra Noormohammadi, Mahnoosh Mohammadzadeh-Shahir, Donya Fahmi, Seyed Mohammad Atyabi, Farah Farahani,
Volume 6, Issue 3 (10-2019)
Abstract
Catharanthus roseus is widely cultivated around the world.  Cold atmosphere plasma (CAP) has been used to improve crop plants seed germination, and plant growth. In the present study, three different times of CAP (40, 50 and 60s) were used for the treatment of C. roseus seeds, and the changes in morphological traits, antioxidant enzymes and the genetic content of the treated plants were examined. Cold plasma (50s) markedly raised the seed germination, length of the leaves and the stem length of the plants in comparison with those in the control group. The catalase and peroxidase enzyme activities had the highest value in 60 and 40 s CAP treated plants, respectively. The sequence related amplified polymorphism (SRAP) markers showed the highest degree of genetic diversity in 50s cold plasma treated plants (Ne = 1.388, I = 0.316, He = 0.217, uHe = 0.237 and P % = 50.08). Based on Neighbor Joining, principle coordination analysis (PCoA) and analysis of molecular variance (AMOVA) test, four significantly distant groups were formed. The 40s and 50s cold plasma treated plants stand far from the control plants due to genetic difference. The results indicate that cold atmosphere plasma could be used as an economic and environmentally safe tool in increasing C. roseus growth characteristics in addition to inducing genetic variations.
 

 

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