Mahnaz Tymoori, Hussna Parvizi, , Mojtaba Koosha,
Volume 13, Issue 1 (4-2026)
Abstract
The aim of this study was to investigate the effect of cofactor-bearing chitosan-coated and non-chitosan carbon nanotubes on induction of apoptosis of cervical cancer HELAcell line by measuring the expression of Bax and Bcl-2 genes as well as cell growth and proliferation. MTT test was used for cell proliferation assay. Expression changes of Bax and Bcl-2 genes were analyzed by Real Time PCR. The MTT results showed that the carbon nanotube coated with caffeic acid carrier chitosan and without caffeic acid carrier and crude carbon nanotube and caffeic acid induce apoptosis and cell toxicity. The results showed that caffeic acid with a chitosan-coated carbon nanotube carrier increased Bax expression and decreased Bcl-2 gene expression, so that the expression ratio of Bcl-2 / Bax compared to free caffeic acid to significantly increase. The results show that the death of cancer cells treated with caffeic acid with chitosan-coated carbon nanotube carries an increase in Bcl-2 / Bax expression ratio and thus induces apoptosis in HELA cancer cells. . Also, the use of caffeic acid as a drug and carbon nanotube-coated carbon nanotube carriers of caffeic acid can be considered as a promising strategy in the treatment of cervical cancer.
