Dr Seyed Mehdi Razavi, Mrs Neda Delir, Dr Sareh Abrahimi, Dr Mansour Afshar Mohammadian,
Volume 11, Issue 2 (8-2024)
Abstract
Using the allelopathic property of some plant species is one of the proposed methods to reduce the use of chemical poisons. Anthriscus nemorosa is an aromatic plant belonging to the Apiaceae that grows naturally in the western and northern parts of in this study. Considering that so far the biochemical and allelopathic properties of this plant have not been investigated in Iran, in this study, the roots of this plant were collected from Khalkhal county and after drying in the shade, the essential oil was extracted by hydro-distillation. The essential oil was analyzed by GC and GC-MS. 52 compounds were identified from the essential oil, representing 80.21% of the total oil. The main constituents were Myristicin (16.92%), Heptane (12.98%), Elemicin (12.54%), Gamma-Terpinene (12.50%), Decane (3.08%), Beta-Eudesmol (2.95%) and Beta-phellandrene (2.52%) and these compounds were remarkably different with that of A. nemorosa native to Serbia. To investigate the allelopathic effects of this plant, an experimental design was factorial experiment in a completely randomized design with four replications. The results of examining the effects of n-hexane, dichloromethane and methanolic extracts (with concentrations of 0, 0.001, 0.01, 0.1 and 1 mg/ml) of the root of this plant on lettuce (Lactuca sativa L. var.capitata) showed that all three extracts had an inhibitory effect on the germination percentage (%), shootlet growth and rootlet growth of lettuce. However, the hexane extract in all treated concentrations had a more inhibitory effect on the germination and shootlet growth of lettuce seed.
