The role of cholestasis in brain hippocampus trauma in male Wistar rat

Eslimi Esfahani, Delaram; Oryan, Shahrbanoo; iNabiun, Mohammad; Karimian Peiro, Manijeh

doi:10.21859/acadpub.nbr.4.2.128

Volume 4, Issue 2 (9-2017) nbr 2017, 4(2): 128-136 | Back to browse issues page

‎ 10.21859/acadpub.nbr.4.2.128

‎ 20.1001.1.24236330.1396.4.2.7.0

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Eslimi Esfahani D, Oryan S, iNabiun M, Karimian Peiro M. The role of cholestasis in brain hippocampus trauma in male Wistar rat. nbr 2017; 4 (2) :128-136
URL: http://nbr.khu.ac.ir/article-1-2950-en.html

The role of cholestasis in brain hippocampus trauma in male Wistar rat

Delaram Eslimi Esfahani ^*

, Shahrbanoo Oryan

, Mohammad INabiun

, Manijeh Karimian Peiro

Kharazmi University

Abstract: (6814 Views)

In cholestasis syndrome, the change of bile acids, salts, endotoxins and opioidsis is accompanied with hepaticencephalopathy and brain trauma; therefore, in this study, the histopathological changes of hippocampus after bile duct ligation were investigated. This study was performed on male Wistar rats. The rats were divided into three groups, namely the control, sham and cholestatic groups. After two weeks, the rats were killed under anesth-esia and their brains were dissected with no delay. The specimens were processed routinely and were sectioned into slices of 6-micron thickness. The sections were stained by Hematoxiline-Eosin (H&E) method. Subsequently, they were studied using optical microscope. Necrotic cells, the diminution of cell density and wrinkled chromatic nuclei were observed in hippocampus section. In addition, the thickness of hippocampus was mostly decreased. However, in the sham and control groups no changes were observed. Since endotoxin causes tissue trauma, the increase of en-dotoxin may result in tissue changes in the brain.

Keywords: bile, endotoxin, hepatic encephalopathy, necrosis

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