Volume 6, Issue 4 (1-2020)                   nbr 2020, 6(4): 415-423 | Back to browse issues page

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Khosroshahi S, Salehghamari E, Amoozegar M A, Saffarian P. Screening methods and enzyme profile of agricultural plant-endophytic bacteria. nbr 2020; 6 (4) :415-423
URL: http://nbr.khu.ac.ir/article-1-3284-en.html
Screening methods and enzyme profile of agricultural plant-endophytic bacteria
Samaneh Khosroshahi , Ensieh Salehghamari * , Mohammad Ali Amoozegar , Parvaneh Saffarian
Department of Cell and Molecular Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran , esaleh@khu.ac.ir
Abstract:   (4353 Views)
Nowadays plant endophytic bacteria have found diverse and useful applications in biotechnology; therefore, much attention has been paid to the isolation, identification, and evaluation of these microorganisms. Since the sterilizing plant tissue surfaces from epiphytic bacteria is difficulty, the efficacy of three different screening methods for endophytic bacteria including 1- HClO sterilization, 2- Periodic sterilization (modified tyndallization) and 3- Triton X100 and HClO sterilization, was evaluated in this study. The modified Tyndallization is an innovative method used in this study to appropriately remove the internal spores of epiphytic bacteria, considered to be an obstacle to the isolation of endophytes. Most of the endophytic bacteria were isolated from dicotyledons and leaves. Endophytic bacteria were also studied for the production of different hydrolase enzymes, whereas the protease enzyme was produced in a wide range of endophytic bacteria in greater quantities than other enzymes. The EndoA strain was molecularly identified and found to be 100% similar to Bacillus halotolerans.

 
Keywords: Bacillus halotolerans, chitinase, hydrolytic enzymes, protease, xylanas
Full-Text [PDF 1300 kb]   (1672 Downloads)    
Type of Study: Original Article | Subject: Microbiology
Received: 2019/07/10 | Revised: 2020/02/24 | Accepted: 2019/10/16 | Published: 2020/01/8 | ePublished: 2020/01/8
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