Volume 6, Issue 2 (8-2019)
nbr 2019, 6(2): 131-139 |
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Esfade H, Mirabolghasemi G, Azarnia M. The joint effect of hydro-alcoholic extract of nettle root and metformin on ovarian tissue of diabetic model of Wistar rat. nbr 2019; 6 (2) :131-139
URL: http://nbr.khu.ac.ir/article-1-3062-en.html
URL: http://nbr.khu.ac.ir/article-1-3062-en.html
Kharazmi University , azarnia@khu.ac.ir
Abstract: (7589 Views)
Diabetes mellitus is known to be resistant to insulin, to dysfunction of beta cells and to increase liver glucose production. Poor glucose control during hyperglycemia causes damage to the tissues and creates dangerous consequences, such as infertility. Chronic hyperglycemia has harmful effects on the growth of follicles, which is essential for normal female sexual function. Metformin is the most widely prescribed drug in diabetes, but chemical drugs, despite their undeniable benefits, have destructive effects, so alternative strategies for current modern diabetes medications are essential. Herbal medicines are widely used by patients, based on non-medical recommendations and as blood-glucose-lowering agents, including the nettle. Nettle contributes to lower plasma glucose levels by secreting insulin and increasing the proliferation of beta cells in the pancreas. Therefore, the aim of this study was to determine the effect of nettle extract as a supplement of metformin on ovarian tissue of diabetic model. In this experimental study, 30 female Wistar rats were used. Animals were weighed and randomly divided into 5 groups (n=6). 1) control group 2) diabetic group who were diabetic with intravenous injection of alloxan (150 mg / kg) 3) diabetic group + Nettle root extract (150 mg / kg) 4) diabetic + metformin (150mg / kg) 5) Diabetic group + Metformin(150 mg / kg) + Nettle root extract(150 mg / kg). At the end of treatment, the effect of metformin and nettle root extract on ovarian tissue and biochemical factors such as blood glucose and sex hormones were compared and the data obtained were analyzed by SPSS. Hyperglycemia and body weight loss after metformin and nettle root increased for 4 weeks. Simultaneous administration of metformin and extracts of nettle root significantly increased the primordial, primary, secondary, and corpus luteum and reduced the atretic follicles and significantly increased FSH, LH and testosterone levels as compared with metformin alone. The results of this study showed that the root of nettle with its antioxidant compounds and other properties could be a complement to metformin with a corrective effect on hyperglycemia and the improvement of ovarian disorders.
Type of Study: Original Article |
Subject:
Animal Biology
Received: 2018/01/13 | Revised: 2019/09/24 | Accepted: 2019/06/11 | Published: 2019/07/30 | ePublished: 2019/07/30
Received: 2018/01/13 | Revised: 2019/09/24 | Accepted: 2019/06/11 | Published: 2019/07/30 | ePublished: 2019/07/30
References
1. Abdi, SH., Salehnia, M. and Hosseinkhani, S. 2013. Comparison of survival and developmental rates of mouse ovarian follicles after two and three dimensional cultures. - J. Modares Med. Sci. 16: 51-63.
2. Abrol, S.O., Trehan, A. and Katare, O.P. 2005. Comparative study of different silymarin formulations: formulation and in vitro/in vivo evaluation. - Curr. Drug Deliv. 2: 45-51. [DOI:10.2174/1567201052772870]
3. Akiniola, O., Gabriel, M., Suleiman, A.A. and Olorunsogbon, F. 2012. Treatment of alloxan induced diabetic rats with metformin or glitazones is associated with amelioration of hyperglycaemia and neuroprotection. - Open Diabet. J. 5: 8-12. [DOI:10.2174/1876524601205010008]
4. Akman, L., Erbas, O., Akdemir, A., Yavasoglu, A., Taskiran, D. and Kazandi, M. 2015. Levetiracetam ameliorates ovarian function in streptozotocin induced diabetic rats. - Gynecol. Endocrinol. 31: 657-662. [DOI:10.3109/09513590.2015.1032931]
5. Alanbaki, A.A., Mayali, H.M. and Mayali, H.K. 2017. The protective effect of quercetin and hesperidin on etoposide induced toxicity in male rats testicular. - JPSR 9: 1394-1405.
6. Alkatib, S.R., AlAzam, A.H.A. and Habeab, S.A. 2012.The effect of vitamin C on ovary of female white rats treated with kmno4. Histological and physiological study. - Kufa J. Vet. Med. Sci. 3: 1-16.
7. Azarneoshan, F., KhatamsazSaz, S. and Sadeghi, H. 2009. The effects of hydro alcoholic extract of doremaaucheri on blood concentration of gonadotropin and androgen hormones in adult rats. - Armaghane Danesh 14: 65-70.
8. Cho, K., Chung, J.Y., Cho, S.K., Shin, H.W., Jang, I.J., Park, J.W., Yu, K.S. and Cho, J.Y. 2015. Anti-hyperglycemic mechanism of metformin occurs via the AMPK/LXRα/POMC pathway. - Sci. Rep. 5: 8145. [DOI:10.1038/srep08145]
9. Coughlan, K., Valentine, R., Ruderman, N. and Saha, A. 2014. AMPK activation:a therapeutic target for type2 diabetes? - Diabet. Metab. Syndr. Obes. 7: 241-253. [DOI:10.2147/DMSO.S43731]
10. Crasto, W., Rao, P. and Gleeson, H. 2014. The effect of metformin on reproduction-A short review. - J. Endocrinol. Diabet. Obes. 2: 1-7.
11. Dkhil, M.A., Zrieq, R., Al-Quraishy, S. and Abdel Moneim, A.E. 2016. Selenium nanoparticles attenuate oxidative stress and testicular damage in streptozotocin-induced diabetic rats. - Molecules 21: 2-13. [DOI:10.3390/molecules21111517]
12. Golalipour, M.J., Ghafari, S., Kouri, V. and Kestkar, A.A. 2010. Proliferation of the β-cell pancreas in diabetic rats treated with urtica dioica. - Int. J. Morphol. 28: 399-404. [DOI:10.4067/S0717-95022010000200011]
13. Gupta, R.K., Kesari, A.N., Murthy, P.S., Chandra, R., Tandon, V. and Watal, G. 2005. Hypoglycemic and antidiabetic effect of ethanolic extract of leaves of Annona squamosa L. in experimental animals. - J. Ethnopharma. 99: 75-81. [DOI:10.1016/j.jep.2005.01.048]
14. Hunt, L.M., Arar, N.H. and Akana, L.L. 2000. Herbs, prayer, and insulin: use of medical and alternative treatments by a group of Mexican-American diabetic patients. - J. Fam. Pract. 49: 216-223.
15. Kargar Jahromi, H. and Karimi Jashni, H. 2016. Effect of nettle root extract on folliculogenesis and estrogen and progesterone hormones in rats. - IJBR 7: 533-538.
16. Khidr, B.M., El-Sokkary, G.H. and Saleh, S.M. 2017. Study on morphological changes induced by aspartame on liver of normal and diabetic male albino rats. - Histol. Histopathol. 4: 1-8 [DOI:10.7243/2055-091X-4-1]
17. Liu, J., Liu, M., Ye, X., Liu, K., Huang, J., Wang, L., Ji, G., Liu, N., Tang, X., Baltz, J.M., Keefe, D.L. and Liu, L. 2012. Delay in oocyte aging in mice by the antioxidant N-acetyl-L-cysteine (NAC). - Hum. Reprod. 27: 1411-1420. [DOI:10.1093/humrep/des019]
18. Ma, Z., Nguyen, T.H., Huynh, T.H., Do, P.T. and Huynh, H. 2004. Reducti on of rat prostate weight by combined quercetin-finasteride treatment is associated with cell cycle deregulation. - J. Endocrinol. 181: 493-507. [DOI:10.1677/joe.0.1810493]
19. Mamta, S. and Preeti, K. 2013. Interacting effect of co-administration of Urtica dioica leaf extract with two oral antidiabetic agents (glimepiride and metformin) in streptozotocin and DNA induced diabetic rats. - IJUPBS 2: 326-335.
20. Mosallanejad, B., Avizeh, R., Najafzadeh Varzi, H. and Pourmahdi, M. 2013. A comparison between metformin and garlic on alloxan-induced diabetic dogs. - Comp. Clin. Path. 22: 169-174. [DOI:10.1007/s00580-011-1381-6]
21. Mukundi, M.J., Mwaniki, N.EN., Piero, N.M., Murugi, N.J. and Kelvin, J.K. 2017. Potential anti-diabetic effects and safety of aqueous extracts of urtica dioica collected from narok county, Kenya. - Pharm. Anal. Acta 8: 2-8. [DOI:10.4172/2153-2435.1000548]
22. Nabrdalik, M. and Grata, K. 2015. Assessment of antifungal activity of extracts from nettle (Urtica dioica L.) against Alternaria solani. - PECO 9: 474-481.
23. Nathan, DM., Buse, JB., Davidson, MB., Ferrannini, E., Holman, RR. and Sherwin, R. 2009. Medical management of hyperglycemia in type 2 diabetes: a consensus algorithm for the initiation and adjustment of therapy: a consensus statement of the American Diabetes Association and the European Association for the Study of Diabetes. - Diabet. Care. 32: 193-203. [DOI:10.2337/dc08-9025]
24. Nayki, U., Onk, D., Balci, G., Nayki, C., Onk, A. and Gunay, M. 2016. The effects of diabetes mellitus on ovarian injury and reserve: an Experimental study. - Gynecol. Obstet. Invest. 81: 424-429. [DOI:10.1159/000442287]
25. Ota, A. and Ulrih, N.P. 2017.An overview of herbal products and secondary metabolites used for management of type two diabetes. - Front Pharmacol. 6: 1-14. [DOI:10.3389/fphar.2017.00436]
26. Pinent, M., Castell, A., Baiges, I., Montagut, G., Arola, L. and Ardevol, A. 2008. Bioactivity of flavonoids on insulin-secreting cells. - Compr. Rev. Food Sci. Food Saf. 7: 299-308. [DOI:10.1111/j.1541-4337.2008.00048.x]
27. Ruder, E.H., Hartman, T.J., Blumberg, J., and Goldman, M. 2008. Oxidative stress and antioxidants: Exposure and impact on female fertility. - Hum. Reprod. Update 14: 345-357. [DOI:10.1093/humupd/dmn011]
28. Saffari, S., Bahadori, M.H., Sharami, SH., Torabzadeh, P. and Goudarzvand, M. 2015. The relationship between level of vitamin C in follicular fluid and maturation of oocytes and embryo quality in patients undergoing invitro fertilization. - JBUMS 17: 22-27.
29. Salemi, Z., Rafie, E., Taghi Goodarzi, M. and Ghaffari, M. 2016. Effect of metformin, acarbose and their combination on the serum visfatin level in nicotinamide/ streptozocin-induced type 2 diabetic rats. - Iran. Red Crescent Med. J. 18: e23814. [DOI:10.5812/ircmj.23814]
30. Soares, J.M.D., Pereira Leal, A.E.B., Silva, J.C., Almeida, J.R.G.S. and de Oliveira, H.P. 2017. Influence of flavonoids on mechanism of modulation of insulin secretion. - Pharmacog. Mag. 13: 639-646. [DOI:10.4103/pm.pm_87_17]
31. Song, R. 2016. Mechanism of metformin: A Tale of Two Sites. - Diabet. Care. 39:187-189. [DOI:10.2337/dci15-0013]
32. Tirabassi, G., Chelli, F.M., Ciommi, M., Lenzi, A. and Balercia, G. 2016. Influence of the hypothalamicpituitary-adrenal axis dysregulation on the metabolic profile of patients affected by diabetes mellitus associated late onset hypogonadism. - Nutr. Metab. Cardiovasc. Dis. 26: 53-59. [DOI:10.1016/j.numecd.2015.10.006]
33. Unal, D., Aksak, S., Halici, Z., Sengul, O., Polat, B. and Unal, B. 2011. Effects of diabetes mellitus on the rat liver during the postmenopausal period. - J. Mol. Histol. 42: 273-287. [DOI:10.1007/s10735-011-9331-9]
34. Vukovic, M., Lapcevic, M., Kalezic, N. and Gvozdenovic, B.S. 2007. The effect of metformin on fasting and postprandial insulin secretion in obese patients with diabetes mellitus type 2. - Srp. Arh. Celok. Lek. 135: 447-52. [DOI:10.2298/SARH0708447V]
35. Wang, C., Makela, T. and Adlercreutz, H. 1994. Lignans flavonoids inhibit aromataz in human prediadiposit. - J. Steroid Biochem. Mol. Biol. 50: 205-212. [DOI:10.1016/0960-0760(94)90030-2]
36. Wolska, J., Czop, M., Jakubczyk, K. and Janda, K. 2016. Influence of temperature and brewing time of nettle (Urtica dioica L.) infusions on vitamin C content. - Rocz. Panstw. Zakl. Hig. 67: 367-371.
37. Woods, Y.L., Petrie, J.R. and Sutherland, C. 2009. Dissecting insulin signaling pathways: Individualized therapeutic targets for diagnosis and treatment of insulin resistant states. - Endocr. Metab. Immun. Disord. Drug Targets 9: 187-198. [DOI:10.2174/187153009788452408]
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