Volume 5, Issue 4 (3-2019)
nbr 2019, 5(4): 379-388 |
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Keypour S, Riahi H, Ebadi M, Borhani A, Asef Shayan M R, Safaie N. The Study of the genetic diversity of two laccate species of Ganoderma lucidum and Ganoderma resinaceum using RAPD marker. nbr 2019; 5 (4) :379-388
URL: http://nbr.khu.ac.ir/article-1-2970-en.html
URL: http://nbr.khu.ac.ir/article-1-2970-en.html
Somayeh Keypour 
, Hossein Riahi , Mostafa Ebadi * , Ali Borhani , Mohammad Reza Asef Shayan , Naser Safaie
, Hossein Riahi , Mostafa Ebadi * , Ali Borhani , Mohammad Reza Asef Shayan , Naser Safaie
, ebadi2023@yahoo.com , m.ebadi@azaruniv.edu
Abstract: (5831 Views)
The genus Ganoderma belongs to Polyporales, well-known for its medicinal properties and as a plant pathogen. The aim of this study was to identify the genetic variation between populations of two Ganoderma lucidum Karst. complex isolates using RAPD. For this purpose, four populations of G. resinaceum Boud. and eight populations of G. lucidum collected and purified during 2011-2012, were studied for the first time in Iran. Ten random primers were used for RAPD reaction. The result for RAPD-PCR studies of G. lucidum complex showed a wide range of diversity, as scores of 61.48 and 40.16 were estimated for the inter-specific diversity of G. lucidum and G. resinaceum, respectively. Cluster analysis confirmed the separation of two species populations in different clusters. In the analysis of genetic structure, two probable groups (K = 2) were identified in the studied germplasm which was also confirmed by the results of bar plot.
Keywords: cluster analysis, genetic structure analysis, genetic variation, germplasm, random primers
Type of Study: Original Article |
Subject:
Plant Biology
Received: 2017/09/27 | Revised: 2019/07/20 | Accepted: 2018/09/25 | Published: 2019/03/18 | ePublished: 2019/03/18
Received: 2017/09/27 | Revised: 2019/07/20 | Accepted: 2018/09/25 | Published: 2019/03/18 | ePublished: 2019/03/18
References
1. Ariffin, D., Idris, A.S. and Singh, G. 2000. Status of Ganoderma in oil palm. – In: Flood, J. (ed.), Ganoderma diseases of perennial crops. – CABI Bioscience, Egham, UK. pp: 49-68. [DOI:10.1079/9780851993881.0049]
2. Cao, Y. and Yuan, H.S. 2013. Ganoderma mutabile sp. nov. from southwestern China based on morphological and molecular data. – Mycol. Prog. 12: 121-126. [DOI:10.1007/s11557-012-0819-9]
3. Cao, Y., Wu, S.H. and Dai, Y.C. 2012. Species clarification of the prize medicinal Ganoderma mushroom "Lingzhi". – Fungal Divers. 56: 49-62. [DOI:10.1007/s13225-012-0178-5]
4. Chang, C.Y., Yeh, Z.Y. and Lee-Chen, G.J. 1996. Analysis of genetic diversity of two intersterility groups of Ganoderma australe by DNA sequencing. –Biol. Bull. Natl. Taiwan Normal Univ. 31: 47-53.
5. Ebadi, M., Riahi, H. and Zare, R. 2014. Genetic diversity of Fusarium semitectum isolates from rice, using RAPD and REP-PCR markers. – Mycol. Iran 1: 19-26.
6. Evanno, G., Regnaut, S. and Goudet, J. 2005. Detecting the number of clusters of individuals using the software structure: A simulation study. – Mol. Ecol. 14: 2611-2620. [DOI:10.1111/j.1365-294X.2005.02553.x]
7. Fu, J.J., Mei, Z.Q., Tania, M., Yang, L.Q., Cheng, J.L. and Khan, M.A. 2015. Development of RAPD-SCAR markers for different Ganoderma species authentication by improved RAPD amplification and molecular cloning. – Genet. Mol. Res. 14: 5667-5676. [DOI:10.4238/2015.May.25.19]
8. Furtado, J.S. 1965. Ganoderma colossum and the status of Tomophagus. – Mycologia. 979-984. [DOI:10.2307/3756901]
9. Gell, I., Cubero, J. and Melgarejo, P. 2007. Two different PCR approaches for universal diagnosis of brown rot and identification of Monilinia spp. in stone fruit trees. – J. Appl. Microbiol. 103: 2629-2637. [DOI:10.1111/j.1365-2672.2007.03495.x]
10. Gottlieb, A.M. and Wright, J.E. 1999 a. Taxonomy of Ganoderma from southern South America: subgenus Ganoderma. – Mycol. Res. 103: 661-673. [DOI:10.1017/S0953756298007941]
11. Gottlieb, A.M. and Wright, J.E. 1999 b. Taxonomy of Ganoderma from southern South America: subgenus Elfvingia. – Mycol. Res. 103: 1289-1298. [DOI:10.1017/S095375629800848X]
12. Gottlieb, A.M., Saidman, B.O. and Wright, J.E. 1998. Isoenzymes of Ganoderma species from southern South America. – Mycol. Res. 102: 415-426. [DOI:10.1017/S0953756297005352]
13. Hammer, O., Harper, D.A.T. and Ryan, P.D. 2012. PAST: paleontological statistics software package for education and data analysis. – Palaeontol. Electronica. 4 (art. 4): 9.
14. Hong, S.G., Jeong, W. and Jung, H.S. 2002. Amplification of mitochondrial small subunit ribosomal DNA of polypores and its potential for phylogenetic analysis. – Mycologia 94: 823-833. [DOI:10.1080/15572536.2003.11833176]
15. Hseu, R.S., Wang, H.H., Wang, H.F. and Moncalvo, J.M. 1996. Differentiation and grouping of isolates of the Ganoderma lucidum complex by random amplified polymorphic DNA-PCR compared with grouping on the basis of internal transcribed spacer sequences. –Appl. Environ. Microbiol. 62: 1354-1363.
16. Keypour, S., Riahi, H., Safaie, N. and Borhani, A. 2014. Mycelial Growth Rate and Macro-and Micromorph-ological Characteristics of Medicinal Species of Genus Ganoderma (Higher Basidiomycetes) from Iran. – Int. J. Med. Mushrooms. 16: 365-374. [DOI:10.1615/IntJMedMushrooms.v16.i4.70]
17. Mei, Z., Yang, L., Khan, M.A., Yang, M., Wei, C., Yang, W. and Fu, J. 2014. Genotyping of Ganoderma species by improved random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) analysis. – Biochem. Syst. Ecol. 56: 40-48. [DOI:10.1016/j.bse.2014.04.012]
18. Miller, R.N.G., Holderness, M., Bridge, P.D., Chung, G. F. and Zakaria, M.H. 1999. Genetic diversity of Ganoderma in oil palm plantings. – Plant. Pathol. 48: 595-603. [DOI:10.1046/j.1365-3059.1999.00390.x]
19. Moncalvo, J.M., Wang, H.F. and Hseu, R.S. 1995a. Gene phylogeny of the Ganoderma lucidum complex based on ribosomal DNA sequences. Comparison with traditional taxonomic characters. – Mycol. Res. 99: 1489-1499. [DOI:10.1016/S0953-7562(09)80798-3]
20. Moncalvo, J.M., Wang, H.H. and Hseu, R.S. 1995b. Phylogenetic relationships in Ganoderma inferred from the internal transcribed spacers and 25S ribosomal DNA sequences. – Mycologia 87: 223-238. [DOI:10.1080/00275514.1995.12026524]
21. Moncalvo, J.M. and Ryvarden L. 1997. A nomenclatural study of the Ganodermataceae Donk. – Synopsis fungorum, Oslo-Norway. pp: 11-114.
22. Moncalvo, J.M. 2000. Systematics of Ganoderma2. – In: Flood, J. (ed.), Ganoderma diseases of perennial crops. 23-49. -CABI
23. Bioscience, Egham, UK. Moradali, M.F., Hedjaroude, G.A., Mostafavi, H., Abbasi, M., Ghods, S. and Sharifi-Tehrani, A. 2007. The genus Ganoderma (Basidiomycota) in Iran. –Mycotaxon 99: 251-269.
24. Park, Y.J., Kwon, O.C., Son, E.S., Yoon, D.E., Han, W., Nam, J.Y. and Lee, C.S. 2012a. Genetic diversity analysis of Ganoderma species and development of a specific marker for identification of medicinal mushroom Ganoderma lucidum. – Afr. J. Microbiol. Res. 6: 5417-5425.
25. Park, Y.J., Kwon, O., Son, E.S., Yoon, D.E., Han, W., Yoo, Y.B. and Lee, C.S. 2012b. Taxonomy of Ganoderma lucidum from Korea based on rDNA and partial β-tubulin gene sequence analysis. –Mycobiology 40: 71-75. [DOI:10.5941/MYCO.2012.40.1.071]
26. Postnova, E.L. and Skolotneva, E.S. 2010. Ganoderma lucidum complex: Some individual groups of strains. – Microbiol. 79: 270-276. [DOI:10.1134/S0026261710020219]
27. Ryvarden, L. 1994. Can we trust morphology in Ganoderma? – In Systematics, Phytopathology and Pharmacology. 5th Int Mycol Congress (eds. P.K. Buchanan, R.S. Hseu and J.M. Moncalvo), Dept. Agric. Chem., National Taiwan University, Taipei, China: 19-24.
28. Safaie, N., Alizadeh, A., Saidi, A., Rahimian, H. and Adam, G. 2005. Molecular characterization and genetic diversity among Iranian populations of Fusarium graminearum, the causal agent of wheat head blight. Iran – J. Plant. Pathol. 41: 171-189.
29. Shakeel, M., Ilyas, M. and Kazi, M. 2013. Evaluation of synthetic hexaploid wheats (derivative of durum wheats and Aegilops tauschii accessions) for studying genetic diversity using randomly amplified polymorphic DNA (RAPD) markers. – Mol. Biol. Rep. 40: 21-26. [DOI:10.1007/s11033-012-1943-y]
30. Smith, B.J. and Sivasithamparam, K. 2000a. Internal transcribed spacer ribosomal DNA sequence of five species of Ganoderma from Australia. – Mycol. Res. 104: 943-951. [DOI:10.1017/S0953756200002458]
31. Smith, B.J. and Sivasithamparam, K. 2000b. Isozymes of Ganodermas species in Australia. – Mycol. Res. 104: 952-961. [DOI:10.1017/S0953756200002446]
32. Smith, B.J. and Sivasithamparam, K. 2003. Morphological studies of Ganoderma (Ganodermmataceae) from the Australasian Pacific regions. – Aust. Syst. 16: 487-503. [DOI:10.1071/SB02001]
33. Steyaert, R.L. 1972. Species of Ganoderma and related genera mainly of Bogor and Leiden Herbaria. –Rijksherbariam publishing, Leiden. pp: 55-118.
34. Steyaert, R.L. 1975. The concept and circumscription of Ganoderma tornatum. – Trans. Br. Mycol. Soc. 65: 451-467. [DOI:10.1016/S0007-1536(75)80043-X]
35. Takamatsu, S. 1998. PCR applications in fungal phylogeny. – In: P. Bridge, D. Arora, C. Reddy and R. Elander (ed.), Applications of PCR in Mycology: 125-152. CAB International.
36. Wasser, S.P. and Weis, A.L. 1999. Medicinal properties of substances occurring in higher basidiomycetes mushrooms: current perspectives (Review). – Int. J. Med. Mushrooms. 1: 31-62. [DOI:10.1615/IntJMedMushrooms.v1.i1.30]
37. Zakaria, L., Kulaveraasingham, H., Guan, T.S., Abdullah, F. and Wan, H.Y. 2005. Random amplified polymorphic DNA (RAPD) and random amplified microsatellite (RAMS) of Ganoderma from infected oil palm and coconut stumps in Malaysia. – Asia Pac. J. Mol. Biol. Biotechnol. 13: 23-34.
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