Volume 6, Issue 1 (5-2019)                   nbr 2019, 6(1): 1-9 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Eslimi Esfahani D, Oryan S, Nabiuni M, Hosseinynia T S. The effects of bile duct ligation on motor cortex region morphology and aquaporin 4 protein concentration in male Wistar rats . nbr 2019; 6 (1) :1-9
URL: http://nbr.khu.ac.ir/article-1-2692-en.html
The effects of bile duct ligation on motor cortex region morphology and aquaporin 4 protein concentration in male Wistar rats
Delaram Eslimi Esfahani * , Shahrbanoo Oryan , Mohammad Nabiuni , Talieh Sadat Hosseinynia
Kharazmi University , eslimi@khu.ac.ir
Abstract:   (4949 Views)
Impaired motor functions were reported in cholestatic animals. This disorder in the function and death of motor neurons is highly dependent on changes in the environment around astrocytes and the blood-brain barrier, which is moderated by the aquaporin 4 protein. For this reason, the effects of cholestasis on motor cortex histology and morphology and aquaporin 4 protein levels were investigated in this study. Samples were stained by hematoxylin-eosin method. Histological changes in cortical brain were investigated. The amount of AQP4 protein in control, sham, and experimental groups were tested by immunohistochemistry. The thickness of motor cortex in cholestatic samples increased in comparison with the control and sham groups. Also, cholestasis caused wrinkle chromatic nuclei. On the other hand, tissue necrosis was detected in cholestatic group compared with sham and control groups. Reduction of cells densities in some cortical layers has been observed, which is probably indicative of cholestasis-induced cell death. AQP4 expression significantly decreased in BDL (p <0.05), but not in other groups (P<0.05). In this study, the pathology of motor cortex, which has also been associated with the decrease of neurons, could be considered the cause of motion abnormalities and AQP4 level reduction in cholestatic rats.
 

 
Keywords: cholestasis, immunohistochemistry, motor function, necrosis, wrinkle chromatic nucleit
Full-Text [PDF 1221 kb]   (1384 Downloads)    
Type of Study: Original Article | Subject: Animal Biology
Received: 2016/12/8 | Revised: 2021/06/6 | Accepted: 2018/12/1 | Published: 2019/04/30 | ePublished: 2019/04/30
References
1. Bataveljić, D., Nikolić, L., Milosević, M., Todorović, N. and Andjus, P.R. 2012. Changes in the astrocytic aquaporin-4 and inwardly rectifying potassium channel expression in the brain of the amyotrophic lateral sclerosis SOD1 (G93A) rat model. - Glia 60: 1991-2003. [DOI:10.1002/glia.22414]
2. Buldanlioglu, S., Turkmen, S., Ayabakan, H.B. and Yenice, N. 2005. Nitric oxide, lipid peroxidation and antioxidantdefence system in patients with active or inactive behcet's disease. - Br. J. Dermatol. 153: 526-30. [DOI:10.1111/j.1365-2133.2005.06543.x]
3. Butterworth, R.F., Lalonde, R., Power, C., and Baker G.B. 2009. Dehydroepiandrosterone sulphate improves cholestasis-associated fatigue in bile duct ligated rats. - Neurogastroenterol Motil. 21: 1319-1325. [DOI:10.1111/j.1365-2982.2009.01356.x]
4. Butz, M., Timmermann, L., Braun, M., Groiss, S.J., Wojtecki, L., Ostrowski, S., Krause, H., Pollok, B., Gross, J., Südmeyer, M., Kircheis, G., Häussinger, D. and Schnitzler, A. 2010. Motor impairment in liver cirrhosis without and with minimal hepatic encephalopathy. - Acta Neurol. Scand. 122: 27-35. [DOI:10.1111/j.1600-0404.2009.01246.x]
5. Chaparro-Huerta, V., Rivera-Cervantes, M., Torres-Mendoza, B. and Beas-Zarate, C. 2002. Neuronal death and tumor necrosis factor-a response to glutamateinduced excitotoxicity in the cerebral cortex of neonatal rats. - Neurosci. Let. 333: 95-98. [DOI:10.1016/S0304-3940(02)01006-6]
6. Dhanda, S., Kaur, S. and Sandhir, R. 2013. Preventive effect of N-acetyl-L-cysteine on oxidative stress and cognitive impairment in hepatic encephalopathy following bile duct ligation. - Free Radic. Biol. Med. 56: 204-215. [DOI:10.1016/j.freeradbiomed.2012.09.017]
7. Eslimi Esfahani, D., Oryan, Sh., Nabiuni, M. and Karimian Peiro, M. 2017. The role of cholestasis in brain hippocampus trauma in male Wistar rat. - Nova Biol. Reperta 4: 128-136. [DOI:10.21859/acadpub.nbr.4.2.128]
8. Eslimi Esfahani, D., Oryan, Sh., Nabiuni, M., Karimian Peiro, M. and Gharooni F. 2017. The effect of hepatic impairment on the aquaporin-4 in the paraventricular (PVN) and Supraoptic (SON) nuclei of rat hypothalamus. - Med. Sci. 27: 252-260.
9. Eslimi, D., Oryan, S., Nasehi, M. and Zarrindast, M.R. 2011. Effects of opioidergic systems upon anxiolytic-like behaviors induced in cholestatic rats. - Pharmacol. 670: 180-185. [DOI:10.1016/j.ejphar.2011.08.024]
10. Felipo, V. 2013. Hepatic encephalopathy: effects of liver failure on brain function. - Nat. Rev. Neurosci. 14: 851-858. [DOI:10.1038/nrn3587]
11. Gharooni, F., Oryan, Sh., Nabiuni, M., Eslimi Esfahani, D., Hosseinynia, T.S., Karimian Peiro, M. and Parsa, S. 2015. The histopathological effects of cholestasis on hypothalamic paraventricular and supraoptic nucleus on male wistar rats. - Nova Biol. Reperta 1: 77-85.
12. Gonzalez-Usano, A., Cauli, O., Agusti, A. and Felipo, V. 2014. Pregnenolone sulfate restores the glutamate-nitric-oxide-cGMP pathway and extracellular GABA in cerebellum and learning and motor coordination in hyperammonemic rats. - ACS Chem. Neurosci. 19: 100-105. [DOI:10.1021/cn400168y]
13. Guillermo, L., Lehmann Maria, C., Larocca Leandro, R., Soria Raúl, A. and Marinelli. 2008. Aquaporins: Their role in cholestatic liver disease. - World J. Gastroenterol. 14: 7059-7067. [DOI:10.3748/wjg.14.7059]
14. Haan, R., Lim, J., van der Burg, S.A., Pieneman, A.W., Nigade, V., Mansvelder, H.D. and de Kock, C.P.J. 2018. Neural representation of motor output, context and behavioral adaptation in rat medial prefrontal cortex during learned behavior. - Front Neural Circuits. 1: 12:75. [DOI:10.3389/fncir.2018.00075]
15. Hatsopoulos, G., Harrison, T. and Donoghue, P. 2001. Representations based on neuronal interactions in motor cortex. - Prog. Brain Res.130: 233-44. [DOI:10.1016/S0079-6123(01)30016-X]
16. Jayakumar, V., Valdes, X.Y., Tong, N., Shamaladevi, W. and Gonzalez, M.D. 2014. Sulfonylurea receptor 1 contributes to the astrocyte swelling and brain edema in acute liver failure. - Norenberg Transl. Stroke Res. 5: 28-37. [DOI:10.1007/s12975-014-0328-z]
17. Jover, R., Rodrigo, R., Felipo, V. and Insausti, R. 2006. Brain edema and inflammatory activation in bile duct ligated rats with diet-induced hyperammonemia: a model of hepatic encephalopathy in cirrhosis. - Hepatol. 43: 1257-1266. [DOI:10.1002/hep.21180]
18. Karambaksh, A., Noori Mougahi, M., Hassan Zadeh, Gh. and Tak Zaree, N. 2013. Excitatory and inhibitory effects of nitric oxide on weight, size, and histological changes of rat cerebellum. - Tehran Univ. Med. J. 10: 595-600.
19. Katanoda, K., Yoshikawa, K. and Sugishita, M.A. 2001. Functional MRI study on the neural substrates for writing. Hum. - Brain Mapp. 13: 34-42 [DOI:10.1002/hbm.1023]
20. Kwiatkowski, A.P. and Mcgill, J.M. 1998. Cholestatic liver diseases in adults. - Gastroenterol. 93: 689-691. [DOI:10.1111/j.1572-0241.1998.206_a.x]
21. Lamireau, T., Zoltowska, M., Levy, E., Yousef, I., Rosenbaum, J., Tuchweber, B. and Desmoulière A. 2003. Effects of bile acids on biliary epithelial cells: proliferation, cytotoxicity, and cytokine secretion. - Life Sci. 72: 1401-1411. [DOI:10.1016/S0024-3205(02)02408-6]
22. Lazeyras, F., Spahr, L., DuPasquier, R., Delavelle, J., Burkhard, P., Hadengue, A., Hochstrasser, D., Mentha, G., Giostra, E, Terrier, F. and Vingerhoets, F. 2002. Persistence of mild parkinsonism after liver transplantation in patients with preoperative minimal hepatic encephalopathy: a study on neuroradiological and blood manganese changes. - Transpl. Int. 15: 188-195. [DOI:10.1111/j.1432-2277.2002.tb00150.x]
23. Leke, R., Oliveira, D.L., Mussulini, B.H.M. and Pereira, M.S. 2012. Impairment of the organization of locomotor and exploratory behaviors in bile duct-ligated rats. - PLoS One 7: 1-8. [DOI:10.1371/journal.pone.0036322]
24. Lindenbach, D. and Bishop, Ch. 2013. Critical involvement of the motor cortex in the pathophysiology and treatment of Parkinson's disease. - Neurosci. Biobehav. Rev. 1838: 1-14.
25. Magen, I., Avraham, Y., Ackerman, Z. and Vorobiev, L. 2009. Cannabidiol ameliorates cognitive and motor impairments in mice with bile duct ligation. -J. Hepatol. 51: 528-534. [DOI:10.1016/j.jhep.2009.04.021]
26. Mechtcheriakov, S., Graziadei, I.W., Kugener, A., Schuster, I., Mueller, J., Hinterhuber, H., Vogel, W. and Marksteiner, J. 2006. Motor dysfunction in patients with liver cirrhosis: impairment of handwriting. - J. Neurol. 253: 349-56. [DOI:10.1007/s00415-005-0995-5]
27. Mechtcheriakov, S., Graziadei, I.W., Rettenbacher, M., Schuster, I., Hinterhuber, H., Vogel, W. and Marksteiner, J. 2005. Diagnostic value of fine motor deficits in patients with low-grade hepatic encephalopathy. - World J. Gastroenterol. 11: 2777-2780. [DOI:10.3748/wjg.v11.i18.2777]
28. Moayedi, M., Weissman-Fogel, I., Salomons, T. and Crawley, A. 2012. Abnormal gray matter aging in chronic pain patients. - Brain Res. 1456: 82-93. [DOI:10.1016/j.brainres.2012.03.040]
29. Mochizuki, Y., Mizutani, T., Shimizu, T. and Kawata, A. 2011. Proportional neuronal loss between the primary motor and sensory cortex in amyotrophic lateral sclerosis. - Neurosci. Lett. 503: 73-75. [DOI:10.1016/j.neulet.2011.08.014]
30. Moezi, L., Shafaroodi, H., Sarkar, S. and Emami-Razavi, SH. 2006. Involvement of nitrergic and opioidergic systems in the hypothermia induced by cholestasis in rats. - Pathophysiol. 13: 227-232. [DOI:10.1016/j.pathophys.2006.08.001]
31. Nakamura, K., Fox, R. and Fisher, E. 2012. CLADA: cortical longitudinal atrophy detection algorithm. - NeuroImage 54: 278-289. [DOI:10.1016/j.neuroimage.2010.07.052]
32. Nudo Randolph, J. 2006. Mechanisms for recovery of motor function following cortical damage. Curr. Opin. Neurobiol. 16: 638-644. [DOI:10.1016/j.conb.2006.10.004]
33. Oryan, Sh., Nabiuni, M. and Eslimi Esfahani, D. 2017. Effects of cholestasis on aquaporin 4 choroid plexus of male wistar rat. - Animal Research 11: 134-144. [DOI:10.21859/acadpub.nbr.4.2.128]
34. Rivara, C.B., Sherwood, C.C., Bouras, C. and Hof, P.R. 2003. Stereologic characterizationand spatial distribution patterns of Betz cells in the human primary motor cortex. - Anat. Rec. 270: 137-151. [DOI:10.1002/ar.a.10015]
35. Rodríguez-Garay, E.A. 2003. Cholestasis: human disease and experimental animal models. - Ann. Hepatol. 2: 150-158.
36. Ropponen, A., Sund, R., Riikonen, S. and Ylikorkala, O. 2006. Intrahepatic cholestasis of pregnancy as an indicator of liver and biliary diseases: a population-based study. - Hepatol. 43: 723-728. [DOI:10.1002/hep.21111]
37. Sheen, J.M., Huang, L.T., Hsieh, Ch.S. and Chen, Ch.Ch. 2010. Bile duct ligation in developing rats: temporal progression of liver, kidney, and brain damage. - J. Pedi. Surg. 45: 1650-1658. [DOI:10.1016/j.jpedsurg.2009.12.019]
38. Thomson, A.B.R. and Shaffer, E.A. 2008. First principles of gastroenterology, the basis of disease and an approach to management. - Janssen and Ortho. 9: 514-519.
39. Tripodi, V., Contin, M., Fernández, M.A. and Lemberg, A. 2012. Bile acids content in brain of common duct ligated rats. - Ann. Hepatol. 11: 930-934.
40. Weiler, N., Wood, L., Yu, J. and Solla, S.A. 2008. Top-down laminar orga-nization of the excitatory network in motor cortex. - Nat. Neurosci. 11: 360-366. [DOI:10.1038/nn2049]
41. Yasuko, I. and Moon Young, Kim. 2015. Nitric oxide in liver diseases. - Trends Pharmacol. Sci. 36: 524-536. [DOI:10.1016/j.tips.2015.05.001]
42. Zhang, Y., Hong, J.Y., Rockwell, C.E., Copple, B.L., Jaeschke, H. and Klaassen, C.D. 2012. Effect of bile duct ligation on bile acid composition in mouse serum and liver. - Liver Int. 32: 58-69. [DOI:10.1111/j.1478-3231.2011.02662.x]
Add your comments about this article : Your username or Email:
CAPTCHA
Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Creative Commons Licence
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.



© 2024 CC BY-NC 4.0 | Nova Biologica Reperta

Designed & Developed by : Yektaweb