Protective effects of eight weeks interval aerobic exercise on decorin, TGF-β and tumor volume in atypical animal of breast cancer

sotoodeh, vahid; gharakhanloo, reza; khaligh fard, solmaz; khaligh fard, samaneh; alizadeh, alimohammad

Volume 15, Issue 14 (10-2017) RSMT 2017, 15(14): 59-71 | Back to browse issues page

‎ 20.1001.1.22520708.1396.15.14.6.6

Mendeley

Zotero

RefWorks

sotoodeh V, gharakhanloo R, khaligh fard S, khaligh fard S, alizadeh A. Protective effects of eight weeks interval aerobic exercise on decorin, TGF-β and tumor volume in atypical animal of breast cancer. RSMT 2017; 15 (14) :59-71
URL: http://jsmt.khu.ac.ir/article-1-236-en.html

Protective effects of eight weeks interval aerobic exercise on decorin, TGF-β and tumor volume in atypical animal of breast cancer

Vahid Sotoodeh

, Reza Gharakhanloo

, Solmaz Khaligh fard

, Samaneh Khaligh fard

, Alimohammad Alizadeh

Abstract: (5066 Views)

Introduction: The decorin is a myokine that modulates the growth and the development of the cancer cells through the interaction with TGF-β. The present study is aimed to evaluate the protective effects of 8 weeks interval aerobic exercise on the expression of decorin and TGF-β, and tumor volume in a typical animal of breast tumor. Materials and Methods: In a exprimental research, forty adult female Balb/c mices were randomly divided into four groups: control, tumor, exercise, and exercise with the tumor. The main exercise was performed four weeks before and after tumor with 50-70 percent of the maximum power of mices. The mices were cancerous by subcutaneous surgery with tumor of adenocarcinoma of mice breast in the tumor group and the exercise group with the tumor. Tumor growth was measured weekly. At the end of the study, tumor and soleus muscle were extracted by surgery. Real-Time PCR method was used to evaluate the expression of decorin and TGF-β and data were analyzed by t-test, one-way ANOVA and post-hoc methods with considering the significant level of p<0.05. Results: The tumor growth rate in tumor group was significantly higher than the exercise group with the tumor. A significant difference confirmed between four groups In the level of decorin expression in soleus muscle (F=12.30 , P=0.0023). The Post hoc test showed that 8 weeks of interval aerobic training significantly increased the expression of decorum gene in soleus muscle in two pairs of exercise group compared with control group and exercise group with tumor compared to tumor group (P=0.02). The expression of TGF-β in tumor tissue showed a significant reduction in exercise group with tumor compared with tumor group. Conclusion: Our results showed that interval aerobic training probably contributes to decreasing the growth of breast cancer cells by increasing the expression of decorin and decreasing the expression of TGF-β.

Keywords: Exercise, Tumor Volume, Decorin, Cancer

Full-Text [PDF 1243 kb] (3828 Downloads)

Type of Study: Research |
Received: 2017/12/18 | Accepted: 2018/01/10 | Published: 2018/02/4

References

1. Goh, J., Endicott, E., Ladiges, W.C. (2014). Pre-tumor exercise decreases breast cancer in old mice in a distance-dependent manner. American Journal of Cancer Research. 4(4):378-84.

2. Shiri, S., Alizadeh, A.M., Baradaran, B., Farhanghi, B., Shanehbandi, D., Khodayari, S., Tavassoli, A. (2015). Dendrosomal curcumin suppresses metastatic breast cancer in mice by changing m1/m2 macrophage balance in the tumor microenvironment. Asian Pacific Journal of Cancer Prevention, 16(9):3917-22.

3. Chen, X., Lu, W., Zheng, W., Gu, K., Matthews, C. E., Chen, Z.,Zhenj,Y., Shu, X.-O. (2011). Exercise after diagnosis of breast cancer in association with survival. Cancer Prevention Research, canprevres. 4(9):1409-18.

4. Loprinzi, P.D., Cardinal, B.J., Winters-Stone, K., Smit, E., Loprinzi, C.L. (2012). Physical activity and the risk of breast cancer recurrence: a literature review. Paper Presented at the Oncology Nursing Forum. 39(3):269-74.

5. Pedersen, B.K., Febbraio, M.A. (2008). Muscle as an endocrine organ: focus on muscle-derived interleukin-6. Physiological Reviews. 88(4):1379-406.

6. Raschke, S., Eckardt, K., Bjørklund, H.B., Jensen, J., Eckel, J. (2013). Identification and validation of novel contraction-regulated myokines released from primary human skeletal muscle cells. PloS one. 8(4):e62008.

7. Karstoft, Kristian, Pedersen, Bente K. (2016). Skeletal muscle as a gene regulatory endocrine organ. Current Opinion in Clinical Nutrition and Metabolic Care. 19(4):270-5.

8. Henningsen, J., Rigbolt, K.T., Blagoev, B., Pedersen, B.K., Kratchmarova, I. (2010). Dynamics of the skeletal muscle secretome during myoblast differentiation. Molecular & Cellular Proteomics. 9(11):2482-96.

9. Li, Yong, Li, Juan, Zhu, Jinghong, Sun, Bin, Branca, Maria, Tang, Ying, Huard, Johnny. (2007). Decorin gene transfer promotes muscle cell differentiation and muscle regeneration. The American Society of Gene Therapy. 15(9):1616-22.

10. Bi, X.L., Yang, W. (2013). Biological functions of decorin in cancer. Chinese Journal of Cancer. 32(5):266-9.

11. Sofeu Feugaing, D.D.S., Götte, M., Viola, M. (2012). More than matrix: the multifaceted role of decorin in cancer. European Journal of Cell Biology. 92(1):1-11.

12. Leygue, E., Snell, L., Dotzlaw, H., Troup, S., Hiller‐Hitchcock, T., Murphy, L.C., Roughly, P.J., Watson, P.H. (2000). Lumican and decorin are differentially expressed in human breast carcinoma. The Journal of Pathology. 192(3):313-20.

13. Imamura, T., Hikita, A., Inoue, Y. (2012). The roles of TGF-β signaling in carcinogenesis and breast cancer metastasis. Breast Cancer. 19(2):118-24.

14. Bedford, T.G., Tipton, C.M., Wilson, N.C., Oppliger, R.A., Gisolfi, C.V. (1979). Maximum oxygen consumption of rats and its changes with various experimental procedures. Journal of Applied Physiology. 47(6):1278-83.

15. Leandro, C.G., Levada, A.C., Hirabara, S.M., Manhães-de-Castro, R.De- Castro, C,B., Curi, R., Pithon-Curi, T.C. (2007). A program of moderate physical training for Wistar rats based on maximal oxygen consumption. Journal of Strength and Conditioning Research. 21(3):751-6.

16. Isanejad, A., Alizadeh, A.M., Amani Shalamzari, S., Khodayari, H., Khodayari, S., Khori, V., Khojastehnejad, N. (2016). MicroRNA-206, let-7a and microRNA-21 pathways involved in the anti-angiogenesis effects of the interval exercise training and hormone therapy in breast cancer. Life Sciences. 151(15):30-40.

17. Khori, V., Amani Shalamzari, S., Isanejad, A., Alizadeh, A.M., Alizadeh, S., Khodayari, S., Khodayari, H., Shahbazi,S.,Zahedi, A., Sohanaki, H,. Khaniki, M., Mahdian, R., Saffari, M., Fayad, R. (2015). Effects of exercise training together with tamoxifen in reducing mammary tumor burden in mice: Possible underlying pathway of miR-21. European Journal of Pharmacology. 765:179-187.

18. Farhanji, B., Latifpour, M., Alizadeh, A.M., Khodayari, H., Khodayari, S., Khaniki, M., Ghasempour, S. ,(2015). Tumor suppression effects of myoepithelial cells on mice breast cancer. European Journal of Pharmacology. 765:171-8.

19. Farsinejad, S., Rahaie, M., Alizadeh, A.M., Mir-Derikvand, M., Gheisary, Z., Nosrati, H., Khalighfard, S. (2016). Expression of the circulating and the tissue microRNAs after surgery, chemotherapy, and radiotherapy in mice mammary tumor. Tumor Biology. 37(10):14225-34.

20. Betof, A.S., Dewhirst, M.W., Jones, L.W. (2013). Effects and potential mechanisms of exercise training on cancer progression: a translational perspective. Brain, Behavior, and Immunity. 30:75-87.

21. Murphy, E.A., Davis, J.M., Barrilleaux, T.L,, McClellan, J.L., Steiner, J., Carmichael, M.D., Pena, M.M., Hebert, J,R., Green, J.E. (2011). Benefits of exercise training on breast cancer progression and inflammation in C3 (1) SV40Tag mice. Cytokine. 55(2):274-9.

22. Moses, H., Barcellos-Hoff, M H. (2011). TGF-β biology in mammary development and breast cancer. cold spring harbor perspectives in biology. 3(1): a003277.

23. Derynck, R., Akhurst, R.J., Balmain, A. (2001). TGF-β signaling in tumor suppression and cancer progression. Nature Genetics. 29(2):117-29.

24. Chamani, R., Asghari, S.M., Alizadeh, A.M., Eskandari, S., Mansouri, K., Khodarahmi, R., Taghdir, M., Heidari, Z., Gorji, A., Aliakbar, A., Ranjbar, B., Khajeh, H. (2015). Engineering of a disulfide loop instead of a Zn binding loop restores the anti-proliferative, anti-angiogenic and anti-tumor activities of the N-terminal fragment of endostatin: Mechanistic and therapeutic insights. Vascular pharmacology. 72:73-82.

25. Arteaga, C.L. (2006). Inhibition of TGFβ signaling in cancer therapy. Current Opinion in Genetics & Development. 16(1):30-7.

26. Heinemeier, K.M., Bjerrum, S.S., Schjerling, P., Kjaer, M. (2013). Expression of extracellular matrix components and related growth factors in human tendon and muscle after acute exercise. Scandinavian Journal of Medicine & Science in Sports. 23(3). e150-61.

27. Sullivan, B.E., Carroll, C.C., Jemiolo, B., Trappe, S.W., Magnusson, S.P., Dossing, S., Kjear, M., Trappe, T.A. (2009). Effect of acute resistance exercise and sex on human patellar tendon structural and regulatory mRNA expression. Journal of Applied Physiology. 106(2):468-75.

28. Csordas, G., Santra, M., Reed, C.C., Eichstetter, I., McQuillan, D.J., Gross, D., Nugent. M.A., Hajnoczky, G., Iozzo, R.V. (2000). Sustained down-regulation of the epidermal growth factor receptor by decorin a mechanism for controlling tumor growth in vivo. Journal of Biological Chemistry. 275(42):32879-32887.

29. Isaka, Y., Brees, D.K., Ikegaya, K., Kaneda, Y., Imai, E., Noble, N.A., Border, W.A. (1996). Gene therapy by skeletal muscle expression of decorin prevents fibrotic disease in rat kidney. Nature Medicine. 2(4):418-23.

30. Stander, M., Naumann, U., Dumitrescu, L., Heneka, M., Loschmann, P., Gulbins, E., Dichgans, J., Weller, M. (1998). Decorin gene transfer-mediated suppression of TGF-β synthesis abrogates experimental malignant glioma growth in vivo. Gene Therapy. 5(9): 1187–94.