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The aim of this study was to examine of the factorial structure of the Self- Regulation Questionnaire in college physical education class (SRQ-PE). 277 students were selected by cluster random sampling in Urmia universities. The results of confirmatory factor analysis determined 5-factor model for the structure of the SRQ-PE with 71.03% of total variance. The confirmatory factor analysis showed acceptable goodness of fit indices for the data among students. Also, the significant positive relationship of intrinsic and identified regulation with PE-enjoyment and intention to continue of physical activity, confirmed the concurrent validity. The items for all sub-scales have a high internal consistency above of 0.75 of Cronbach's alpha. The findings suggest that SRQ-PE is a valid and reliable measurement for assessing the motivational self-regulation in college PE class. As a conclusion, the results demonstrate the importance of developing self-determination in PE, as it is associated with desirable behavioural outcomes.