Journal of Engineering Geology

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Introduction
In many areas of the world, the mechanical properties of soils for utilization of land are not sufficient. For improvement of these lands, soil stabilization such as compacting, installation of nails, elders of piles, mixing soil with lime or cement before or during constructions on the surface or inside of the ground can be useful. Microbially induced carbonate precipitation (MICP), due to its versatility and stable performance, has been recently attracted the attention of many researchers in the field of the geotechnical engineering around the world. MICP is a biological technique that is naturally caused to create a cementation agent, which is known as calcium carbonate or calcite by controlling the metabolism of bacteria. Although there are many biological processes that can be lead to MICP, but the using of urea hydrolysis by bacteria is commonly used more. In this method, aerobic bacteria with the enriched urease enzymes inject into the soil. Hydrolysis of urea occurs when the bacteria speeds up the hydrolysis reaction to produce ammonium and carbonate ions. In the presence of soluble calcium ions, carbonate ions are precipitated and formed the calcium carbonate crystals. When these crystals are formed on a grain of soil or like a bridge between them, they prevent the movement of grains and thus improve the mechanical and geotechnical properties of the soil.
Material and methods
In the present study, the effect of increasing fines on the improvement of Anzali sandy soil, and soil resistance parameters for improving the clean sand and its mixtures with a fine grained cohesive soil and a fine grained cohesionless soil separately in a percentage weight of 30 by MICP and using a small scale of direct shear test (6×6) have been investigated. In the present study the sandy soil was collected from the coast of Bandar Anzali Free Zone and for the preparation of samples of clayey sand and silty sand, Kaolinite clay soils and Firouzkooh broken silt were used, respectively. Anzali sand is poorly graded and had a rounded corner with an average particle size of 0.2 mm, somewhat, sharpening cores are also found in its granulation. In addition, its fine grained content is very small (less than 1%). The Kaolinite clay is also labeled with a liquid limit of 40, a plastic limit of 25, and a plasticity index of 15 as an inorganic clay (CL). The used microorganism in this study is urease positive Sporosarcina pasteurii, which is maintained with the number of PTCC1645 at the Center Collective of Industrial Microorganisms of Iran Scientific and Research Organization. The bacterium was cultured in a culture medium containing 20 g/l yeast extract and 10 g/l ammonium chloride at pH 9 under aerobic conditions in incubator shaker machine at 150 rpm and temperature of 30 °C. The organism was grown to late exponential/early stationary phase and stored at 4 °C before injection in samples. A solution of calcium chloride and urea with a molar ratio of one is also used as a cementation solution. With the direct shear test (6cm×6cm) as a benchmarking of the shear strength in the before and after improvement steps, molds fitted with a shear box made of the galvanized sheet with a thickness of 0.6 mm and it consists of two main parts, the body,  in the middle of which an exhaust pipe was embedded in the injector waste fluid. At the bottom of the samples, a layer of filter paper was placed in order to prevent soil washes, and then all samples with a thickness of 2 cm, with a relative density of 30% at the same weight and height were pressed. In the upper part of the samples, a layer of filter paper is similarly used to prevent the discontinuity of soil particles when injected biological materials are used. Biological solutions are injected from the top to the specimens and allowed to penetrate under the influence of gravitational and capillary forces in the sample and discharge the inhaled fluid from the exhaust pipe. The criterion for determining the volume of the solution to inject into each sample is the pure volume (PV) of soil. The preparation process of the samples was initiated by injection of a PV water unit, followed by a two-layer mixture of bacterial suspensions and cementation solutions, each with a volume of one PV, and then for biological reactions, 24 hours to the sample at laboratory temperature (25 ± 2) is given. After the time of incubation, the solution of cementation is injected into the sample for a period of three days and every 24 hours. The processing time of samples is also considered 28 days. In this study, optical density (OD) was selected as a benchmark for estimating the concentration of bacterial cells in the culture medium, and in all stages of development, and precisely before injection of bacteria suspension into soil samples, it was measured by a spectrophotometer device at 600 nm (OD₆₀₀) wavelength, which was obtained for all bacterial suspensions in the range of 1.7 to 2 before the injection. To determine the activity of urea bacteria, 1 ml of bacterial suspension was added to nine milliliters of 1.11 molar urea solution, and by immersing the electrode of the electrical conductivity in the solution, its conductivity was recorded for 5 minutes at 20 ± 2 ° C. The rate of urea activity in the pre-treatment stage for all specimens was in the range of 0.8 to 1.23 mS min^-1. In order to evaluate the shear strength parameters of soil samples, before and after the improvement operations, a direct shear test was used based on the ASTM D3080 standard. This test was performed for all samples under stresses of 50, 100 and 150 kPa in undrained conditions at a loading speed of 1 mm/min up to a strain of 15%. Also, samples of soil with a moisture content of 7% and a relative density of 30% (as already mentioned) have been restored. SEM analysis was carried out to determine the distribution of sediment between soil particles and EDX analysis in order to identify carbonate calcium sediment formation elements in improved soil samples, by scanning electron microscopy on Anzali sandy soil samples in before and after improvement conditions.
 
 
Conclusions
The effect of the increasing cohesive and cohesionless fines on the bio-treated process of sandy soil is the main subject of this research. For this purpose, three samples of clean sand, sand containing 30% clay and sand mixture with 30% silt in a relative density of 30% were treated with MICP method and their shear strength parameters were evaluated by direct shear test after 28 days of processing. Using the direct shear test and analyses of SEM and EDX data, the results are represented as below:
1.  The microbial sediment of carbonate calcium has greatly improved the resistance properties of all three soil samples.
2. A sample of clayey sand, in spite of a higher improvement compared to the other samples with an average shear strength of 113.7% in comparison to to its untreated state, it has the lowest shear strength among the three improved samples.
3. Increasing the clay content of 30% increases the soil voids. On the other hand, it reduces the friction angle and shear strength of the soil in the pre-treated state and also facilitates easier movement of the bacteria between the pores in the soil. More favorable distribution of sediment calcium carbonate was occurred and, as a result, increased adhesion between soil particles.
4. The increase of cohesionless fine particles creates more bonding points between sand particles and, therefore, calcium carbonate crystals form shorter distances between the soil bridges. As a result, with the end of the improvement process, the shear strength parameters of the sandy soil containing 30% of the silt compared to the clean sand have a higher value.
5. SEM images of the clean sand in both before and after improvement show that the calcium carbonate precipitation occurred with a uniform and thin layer that surrounds sand grains and another part of the sediments formed in the joint of grains.
6. Cube-shaped crystalline sediments confirm that the sediment formed in the soil is a stable type of calcite and that the relative increase in the friction angle of the improvement samples can be attributed to solid particles and multifaceted sediments. Also, the elements of carbon, oxygen, and calcium, which are the main components for the formation of calcium carbonate deposits, have been found in the EDX analysis of improvement sand samples../files/site1/files/124/7sohrabi%DA%86%DA%A9%DB%8C%D8%AF%D9%87.pdf
 

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Introduction
Series A of coarse-grained alluvial deposits of Tehran are extended in eastern and north-eastern areas of Tehran. Analyzing and studying of these alluvial deposits from a geological point of view as well as their creation time and general characteristics such as the deposits’ mineral types, their source, and formation conditions, gives a better point of view to geotechnical engineers about exploring their characteristics as well as geotechnical aspects in underground structure design, excavations, and foundation design processes. On the other hand, in order to analyze stability, estimating the factor of safety and the seismic design of these structures, considering their location, which is in Tehran with a high seismic hazard area, the necessity of knowing the exact mechanical and dynamic properties of Tehran's alluvium is felt more than ever.
Material and methods
Due to the grain size of Tehran’s coarse-grained alluviums (series A) as well as high level of cementation of them, it is impossible (or maybe so difficult) to make undisturbed samples in order to do experiments. Such that it is excavated 23 boreholes with 30 to 140 meters depth as well as 17 test wells with 20 meters depth in an area which was extended in 10 kilometers in long which were located in Tehran’s No. 13 and No. 14 districts (as it can be seen in Figure 1). During the excavation of the entrance ramp and tunnel of eastern highway of Tehran, in-situ tests have been done in different sequences. Since it was important to investigate real behavior of these alluviums, different in-situ tests such as plate load test, in-situ shear test, pressuremeter test, and downhole test have been done as well as many laboratory and field tests. Furthermore, (1) X-Ray Diffraction (XRD) and (2) X-ray Fluorescence (XRF) as well as (3) Scanning Electron Microscopy (SEM) methods, have been used to explore the type of minerals and those used in cementation.
 
 
 
 

(ب)
 
 
 
Figure 1. a) Geological plan and the location of boreholes and test wells in the alignment of East Tehran Freeway
Results and discussion
Based on the results of XRD tests, it is quite clear that the largest weight percentages of tested samples are lime and silica.
Calcium and magnesium levels-as the high-power cations in flocculation process-in soil sample No. 1 (soil with high cementation level) are much more than soil sample No. 2 (soil with moderate cementation level).
This is the cause of high cementation level of soil sample No. 1 comparing with soils sample No. 2. A rapid increase in stress level can be seen in in-situ shear test results, in low shear displacements, up to reaching a maximum of τ_p (peak point) and afterwards reduction in shear stress with softening behavior.  
Cohesion and shear strength levels also increase by increasing the depth. According to the plate load tests results, an increase in soil modules changes can be seen in different depths by depth increasing.
Large tendencies to increase in volume and dilation can be seen in under shear load cemented soils, after applying a primary compression on them. A brittle behavior with the occurrence of a certain peak can be seen in cemented samples. The significant increase in strength is directly related to the severe dilation rate, which can be seen in cemented samples results.  The shear strength would be decreased, if this cement is broken during the particles’ displacements.
The results of downhole tests are shown in Figure 2. According to this figure, it has been explored that V_s,30 is about 600 m/s in moderate cemented soils while it is about 850 m/s in highly cemented soils.  Because of the homogeneity and uniformity of sedimentary deposits, shear wave velocity is increasing due to the higher density of the layers and high level of cementation in both of the soil types. However, this increase is not significant at depths above 25 meters.
Conclusion
Based on the results, cementation level of the eastern coarse-grain-alluvium of Tehran is moderate to high and minerals used in cementation of this type of soil are generally carbonated and especially calcite.
Investigating the level of cementation of soil as well as the results of chemical analysis and in-situ tests, it can be found that the strength and deformation parameters of the soil are directly related to the degree of its cementation.
Based on the obtained results, the deformation modulus increases by about 25%, the cohesion by about 55% and the shear wave velocity by about 30% with increasing the degree of cementation (Table 1).
Increases of these parameters are directly related to depth. However, the cementation level does not significantly affect the internal friction angle of the soil.
Table 1. Average results of in-situ shear tests

Deformation Modulus (MPa)	Peak Friction Angle (deg.)	Cohesion (kPa)	USCS	Depth (m)	Sample
50-60	39	30-35	GW-GM	5	Moderately Cemented Soil (M.C. Soil)
75-85	41	50-60	SP-SC	10
85-90	41	50-60	GW-GC	15
95-105	41	50-60	GW-GC	20
60-70	39	35-40	GW-GM	5	Highly Cemented Soil (H.C. Soil)
75-85	39	50-60	GW-GC	10
110-120	42	65-75	GW-GC	15
125-140	41	110-120	GC	20