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 The aim of this study was to investigate the psychometric properties of Persian language version of the ambivalence over emotional expression questionnaire (AEQ). Translation-back translation was used to prepare the Persian version of AEQ. A convenience sample of 521 students from three universities in Tehran in 2015-2016 was examined.  We assessed validity of the questionnaire by  construct validity (via exploratory factor analysis and confirmatory factor analysis) and convergent validity. Cronbach alpha, split half reliability, test-retest reliability coefficients were used for assessing reliablity. Exploratory factor analysis of AEQ’s items showed two factors named ambivalence over expression of positive emotions and ambivalence over expression of negative emotions. Confirmatory factor analysis confirmed the factor structure resulted from exploratory factor analysis which was different from the proposed factors in original questionaire. For total questionnaire and two extracted factors, Cronbach alphas were between 0.77 and 0.86, the Spearman–Brown coefficients were between 0.77 and 0.88 and the test- retest reliability coefficients were between 0.72 and 0.79. The results of this study indicated that this questionnaire have  appropriate psychometric properties among Iranian Population.