Kharazmi University Research in Sport Medicine and Technology 2252-0708 2588-3925 21 26 2023 12 1 The Effect of Aerobic Exercise and Quercetin Supplementation on Myostatin and Follistatin Levels in the Heart Tissue of Rats with Type 2 Diabetes 1 17 FA Mohsen Soltani Payame Noor University ali_barzegari_1014@yahoo.com N Ali Barzegari Payame Noor University ali_barzegari@pnu.ac.ir Y 0000-0001-7926-5885 Saeed Naghibi Payame Noor University sdnaghibi@yahoo.com N 0000-0001-5591-1678 Mohammad hassaan Dashty khavidaki Payame Noor University Dashty54@gmail.com N 0000-0002-5693-9442 10.61186/jsmt.21.26.1 This study aimed to investigate the effect of quercetin supplementation and training on myostatin and follistatin levels in the heart tissue of diabetic rats. Methods: For this purpose, 50 10-week-old male Wistar rats with an average weight of 237±33 grams were randomly divided into five groups of 10 including: healthy control, diabetes, diabetes+supplement, diabetes+exercise, and diabetes+supplement+exercise. . The training protocol includes five sessions per week for eight weeks on a treadmill without a special slope. The rodents ran for 60 minutes at a speed of 25 meters per minute in each session.and the supplement groups received 50 mg/kg quercetin daily by gavage. The results showed that the difference in myostatin levels among the groups is significant (P=0.001). There was a significant decrease in myostatin levels between exercise+supplement+diabetes group with diabetes (P=0.002). There was also a significant difference between the supplement+diabetes and exercise+diabetes groups (P=0.009). Also, the results showed that the difference in follistatin levels among the groups is significant (P=0.001). The results showed that follistatin levels in the heart tissue increased significantly between the groups of diabetes, exercise+diabetes, supplement+diabetes and exercise+supplements+diabetes with the control group (respectively: P=0.005; P=0.001; P=0.001; P = 0.001). It seems that moderate intensity aerobic exercise and quercetin supplementation have a positive and beneficial effect on the levels of myostatin and folstatin. Exercise, Diabetes, Follistatin, Myostatin http://jsmt.khu.ac.ir/article-1-528-en.html http://jsmt.khu.ac.ir/article-1-528-en.pdf

Kharazmi University Research in Sport Medicine and Technology 2252-0708 2588-3925 21 26 2023 12 1 The Effect Of High Intensity Aerobic Training On Mitochondrial DNA Copy Number In Visceral Adipose Tissue Of Ovariectomized Rats Fed With High-Fat Diet 18 29 FA elahe talebi-garakani University of Mazandaran talebi_umz@yahoo.com N shokoufeh kheradmand University of Mazandaran UMZ.kheradmand@gmail.com Y khadijeh nasiri University of Mazandaran khadijeh_nasiri@yahoo.com N 10.61186/jsmt.21.26.18 The prevalence of obesity in postmenopausal women is higher compared to men. A decrease in estrogen with menopause leads to dysfunction in mitochondria. The purpose of this study is to investigate the effect of high intensity aerobic training(HIT) on the copy number of mitochondrial genome (mtDNAcn) and mitochondrial transcription factor A(Tfam) in visceral adipose tissue(VAT) in ovariectomized(OVX) rats fed with high-fat diet(HFD). 40 rats (8 weeks, 200±20 gr) were divided into 5 groups:1) normal diet (ND), 2) ND+OVX, 3) HFD, 4) HFD+OVX, and 5) HFD+OVX+HIT. HIT performed aerobic training (80-85% of maximum oxygen consumption) for 8 weeks/5 sessions/42 minutes.48 hours after the last training session, VAT samples were taken to evaluate mtDNAcn and Tfam gene expression by qRT-PCR method. ANOVA and LSD tests were used to compare the differences between groups. OVX and HFD didn’t lead to a significant change in Tfam gene expression, but caused a significant decrease in the mtDNAcn compared to the control group. HIT caused a significant increase in Tfam gene expression compared to the control group and Tfam gene expression and mtDNAcn compared to the HFD+OVX. Hence, HIT improves mitochondrial function through mtDNAcn and Tfam gene expression in VAT in OVX rats fed by HFD.   Ovariectomized, Visceral Adipose Tissue, Intense Aerobic Exercise Training, mtDNAcn, Tfam http://jsmt.khu.ac.ir/article-1-587-en.html http://jsmt.khu.ac.ir/article-1-587-en.pdf

Kharazmi University Research in Sport Medicine and Technology 2252-0708 2588-3925 21 26 2023 12 1 Estimate 1 Repetition Maximum Of Active Women Based On The Rating Of Perceived Exertion In Chest Press Movement: Provide a Predictive Qquation 30 40 FA javid shah hosseini shahid beheshti university javid.shahhoseini@gmail.com N arezoo eskandari shahid rajaee university a.eskandari_1988@yahoo.com N 0000-0002-7376-1156 mohamad fashi shahid beheshti university m_fashi@sbu.ac.ir Y 0000-0002-0995-4703 10.61186/jsmt.21.26.30 Using standard tests to assess maximum strength is very important where resistance training planning is based individually. This study aimed to estimate 1 repetition maximum of active women based on the Rating of Perceived and to provide a special predictive equation. 30 healthy women (20-35 years; BMI: 26.32 ± 3.34 kg/m2) performed one repetition of chest press with eyes close and then chose a number to determine the intensity by Rating of Perceived Exertion scale of 6-20. In order to evaluate the validity of the designed equation, one repetition of the maximum number of subjects was calculated by Brzycki equation, and correlation was determined by Pearson's correlation method. There was a high correlation between the present study equation and the Brzycki method (r=0.89) and also after cross validations (r=0.91). No significant difference was observed between the results of the present equation and the results of Berzyski's equation. It seems that the equation of the present study” 1RM={(20-RPE" /" 40) +1 }× weight/2 "  can provide a reliable estimate for 1 repetition maximum of chest press in healthy women. Maximum strength, Resistance training, Rating of Perceived Exertion. http://jsmt.khu.ac.ir/article-1-594-en.html http://jsmt.khu.ac.ir/article-1-594-en.pdf

Kharazmi University Research in Sport Medicine and Technology 2252-0708 2588-3925 21 26 2023 12 1 The Acute Effect Of Warm-Up (General And Post-Activation Potentiation) On Vertical Jump And Jump-Landing Technique Of Handball Players With Trunk Dysfunction 41 55 FA zahra ghahremani University of Guilan z.ghahremani20@gmail.com Y 0000-0003-1678-1983 Hassan daneshmandi University of Guilan daneshmandi-ph@yahoo.com N 0000-0003-1154-0531 mehrdad anbarian Bu-Ali Sina University mehrdadanbarian36@gmail.com N 0000-0002-3605-5586 10.61186/jsmt.21.26.41 The purpose of this study was to assess the acute effect of warm-up protocols (general and post-activation potentiation) on vertical jump performance and jump-landing technique in female handball players with trunk dysfunction. Twelve trained female handball players with trunk dysfunction participated voluntarily in this study. Subjects randomly performed three different warm-up protocols on separate days: 1- general warm-up protocol, 2- general warm-up with 2 repetitions of dynamic half-squat at %90 of one repetition maximum, 3- general warm-up with 2 repetitions of static half-squat at %90 of one repetition maximum. In the fifth minutes of recovery period after 3 protocols, subjects performed vertical jump and the Landing Error Scoring System tests, and Landing Error Scoring System scores, valgus and flexion angles, and vertical jump height was assessed. ANOVA with repeated measures was used for statistical data analysis. The jump height increased significantly after performing the second and third protocols related to general warm-up (p=0.039, p=0.047 respectively), but there weren’t a significant difference in Landing Error Scoring System score, valgus and flexion angles for three protocols. It seems that the special warm-up method by post-activation potentiation can improve vertical jump performance without influencing Anterior cruciate ligament injury risk.   Post-Activation Potentiation, Trunk Dysfunction, Landing, Handball. http://jsmt.khu.ac.ir/article-1-496-en.html http://jsmt.khu.ac.ir/article-1-496-en.pdf

Kharazmi University Research in Sport Medicine and Technology 2252-0708 2588-3925 21 26 2023 12 1 The Effect Of Corrective Exercises Focused On Chain Reactions, On Scapular Position, Proprioception And Shoulder Functional Stability Of Female Students With Upper-Crossed Syndrome 56 76 FA Narmin Ghanizadeh hesar Associate Professor, Department of Exercise Physiology and Corrective Exercises, Faculty of Sport Sciences, Urmia University, Iran n_hesar@yahoo.com Y 0000-0002-4489-6705 Behnam Moradi Department of Sport Injuries and Corrective Exercises, Faculty of Physical Education, Kharazmi University, Tehran, Iran. B_moradi91@yahoo.com N 0000-0001-6803-2947 Marzieh Musa Gholizadeh Fallah Department of Sport Injuries and Corrective Exercises, Urmia Islamic Azad University, Iran. Marzieh.golizadeh75@gmail.com N 0000-0002-2253-4375 10.61186/jsmt.21.26.56  Upper-crossed syndrome is one of the most common disorders of the upper body, which is associated with 45-65% of shoulder pain. This study has been conducted to investigate the effect of corrective exercises focused on chain reactions, on scapular position, proprioception and shoulder functional stability of female students with UCS. 30 female students between 14 and 18 years of age in Urmia city who had the mentioned disorder were selected using the purposive sampling method and were randomly divided into two experimental groups (15 people) and control (15 people). A tape measure was used to evaluate the distance between the median of the sternum to the coracoid ridge and the posterior-lateral distance of the acromion to the thoracic vertebra (scapular position), the Layton flexometer to measure proprioception, the Y Balance Test to evaluate the function of the upper limb, and the goniometer, tape measure and flexible ruler, respectively were used to measure head and shoulder forward angle and kyphosis. Independent and dependent t-tests were used to compare inter-group and intra-group means, respectively. The present study showed that the effect of corrective exercises on scapular position, proprioception and shoulder functional stability of female students with UCS was significant. Scapular Position, Proprioception, Functional Stability, Upper Crossed Syndrome http://jsmt.khu.ac.ir/article-1-553-en.html http://jsmt.khu.ac.ir/article-1-553-en.pdf

Kharazmi University Research in Sport Medicine and Technology 2252-0708 2588-3925 21 26 2023 12 1 Investigating The Effect Of Endurance Training as a Therapeutic Method On Muscle Cachexia and Cardiovascular Consequences caused by Breast Cancer 77 88 FA Elham Shahabpoor Assistant Professor of exercise Physiology, Department of Sports Sciences, University of Hormozgan, Iran. e.shahabpour@hormozgan.ac.ir Y 0000-0002-8316-3547 Mohammad Amin Delavari Assistant Professor of exercise Physiology, Department of Sports Sciences, University of Hormozgan, Iran. Delavari@hormozgan.ac.ir N 0000-0002-7501-8788 10.61186/jsmt.21.26.77 Cachexia syndrome and the risk of cardiovascular diseases are highly prevalent in cancer patients.The purpose of the present study was to investigate the effect of endurance training as a therapeutic method on muscle cachexia and cardiovascular consequences caused by breast cancer.  After 2 weeks of breast cancer induction, 20 BALB/c mice (age: 6-8 weeks and weight: 17- 18 g) mice performed the maximum aerobic capacity test and were randomly allocated into tumor-control and tumor-exercise groups. The tumor-exercise group performed the endurance Training protocol for 6 weeks, 5 days a week with gradual increase in intensity from 12 to 20 (m.min-1) and duration from 25 to 55 minutes And 24 hours after the last training session, the maximum aerobic capacity test was performed. 48 hours after the last training session, the mice were sacrificed. Heart weight, weight of gastrocnemius and soleus muscle, heart mass index, ratio of weight of soleus muscle to body weight and ratio of weight of gastrocnemius muscle to body weight were measured in tumor-control and tumor-exercise groups. Data were analyzed by independent t-test at the significance level of P≥0.05. The results of the present study showed a significant increase in heart weight (P=0.005), spleen weight (P=0.029), weight of the gastrocnemius muscle (P=0.009) and soleus muscle (P=0.022) in the tumor-exercise group compared to the tumor-control group. Also, significant increase in the heart mass index (P=0.016) and the ratio of the weight of the gastrocnemius muscle to the body weight (P=0.005) and the ratio of the soleus muscle to the body weight (P=0.021) were observed in the tumor-exercise group compared to the tumor-control group. In conclusion,Endurance training seems to be a potential treatment method and strategy to improve the health of the skeletal muscle and cardiovascular system and reduce the process of muscle cachexia caused by breast cancer. Endurance Training, Cachexia, Cardiovascular Disease, Breast Cancer. http://jsmt.khu.ac.ir/article-1-606-en.html http://jsmt.khu.ac.ir/article-1-606-en.pdf

Kharazmi University Research in Sport Medicine and Technology 2252-0708 2588-3925 21 26 2023 12 1 The Effect Of Eight Weeks Of Endurance Training On The Expression Of Glutathione Peroxidase 4 And Malondialdehyde In The Soleus Muscle Of Aged Aale Wistar Rats 89 102 FA mojtaba salehpour Shahid Rajaee Teacher Training University salehpour57@gmail.com Y 0000-0002-6208-2502 Ali sarlak Shahid Rajaee Teacher Training University Ali.sarlak.203@gmail.com N 0009-0008-7700-0558 hadi samadi Shahid Rajaee Teacher Training University samadi@gmail.com N 0000-0002-0618-2437 10.61186/jsmt.21.26.89 Introduction: The increase of iron due to aging affects the process of ferroptosis, and the final product of lipid peroxidation, MDA, is the central regulator of ferroptosis. Therefore, the aim of the research was to determine the effect of eight weeks of endurance training on the expression of GPX4 and MDA in the soleus muscle of aged male rats. Materials and Methods: This study is of an experimental type. 20 healthy rats were divided into two groups of 10 each: training group and control group. The training was done incrementally for eight weeks. 48 hours after the last training session, the soleus muscle of mice was surgically removed and the amount of variables was measured by gene expression and ELISA methods. Results: There was no significant difference in the level of GPX4 gene expression in the soleus muscle of exercise group rats compared to control group rats (p=0.4079). But the amount of MDA in the training group was significantly reduced compared to the control group (p=0.0028). Conclusion: According to the findings of the present research, it seems that the effect of endurance training in this research shows a decrease in ferroptosis. The absence of a significant difference in the level of GPX4 gene expression is a sign that endurance training with this intensity does not affect it. Endurance training, aged rat, ferroptosis, GPX4, MDA http://jsmt.khu.ac.ir/article-1-584-en.html http://jsmt.khu.ac.ir/article-1-584-en.pdf

Kharazmi University Research in Sport Medicine and Technology 2252-0708 2588-3925 21 26 2023 12 1 Acute Effect Of Hydro Alcoholic Extract Of Melissa Officinalis On Metabolic Rate, Substrate Oxidation, And Salivary Cortisol Of High-Intensity Interval Exercise In Inactive Women 103 117 FA Mohsen Mohammadnia Ahmadi University of Birjand m.m.ahmadi2005@birjand.ac.ir Y 0000-0002-8073-9774 Razieh Heidari University of Birjand r.heidari9311@yahoo.com N 0009-0001-3179-3774 10.61186/jsmt.21.26.103 Nowadays, the use of traditional herbal remedies for the prevention and treatment of diseases along with exercise has become very popular. The aim of this study was to investigate acute effect of hydro alcoholic extract of melissa officinalis on metabolic rate, substrate oxidation and salivary cortisol during and after high-intensity interval exercise (HIIE) in inactive women. Ten inactive women were selected based on criteria (BMI 22.28±0.60 kg/m2). Individuals participated in two separate sessions. In the first session, extract of melissa officinalis (500 mg) were intake one hour before HIIE. In the second session, the placebo (500 mg of starch) was intake. The HIIE program consisted of 11 alternates (1-minute with 90% vVO2max followed by 2-minute rest intervals with 60% vVO2max). Pre, during and after HIIE, respiratory gases was measured using the respiratory gas analyzer and was used to calculate metabolic rate (Energy Expenditure, VO2) and substrate oxidation (Fat oxidation, Respiratory Exchange Ratio (RER)). Heart rate monitored by polar sensor. Salivary cortisol after collection was measured by chemiluminescence method. Based on results, there was no significant difference in VO2 (P =0.51), Fat Oxidation (P= 0.9), RER (P= 0.76), Heart rate (P= 0.42) and energy expenditure (P=0.6) between melissa officinalis and placebo intake. Also, there was no significant difference in salivary cortisol (P= 0.15). Generally, hydro alcoholic extract of melissa officinalis supplement intake along HIIE has no effect on metabolic rate, substrate oxidation and cortisol level in inactive women.   Cortisol, Melissa Officinalis, High Intensity Intermittent Activity, Respiratory Exchange Ratio, Oxygen Consumption, Energy Expenditure. http://jsmt.khu.ac.ir/article-1-613-en.html http://jsmt.khu.ac.ir/article-1-613-en.pdf

Kharazmi University Research in Sport Medicine and Technology 2252-0708 2588-3925 21 26 2023 12 1 Evaluation Of Pain And Musculoskeletal Disorders In People With A History Of Covid-19 118 128 FA seyed mohammad hosseini University of beheshti moh_hosseini@sbu.ac.ir N 0000-0002-7414-1530 vahid seyedazizi University of Isfahan vahid.seyedazizi@gmail.com N 0000-0001-6424-5750 sahar bala khiyavi University of rasht N 0009-0005-4702-3642 ali keshtiaray Islamic Azad University keshtiarai@yahoo.com Y 0000-0002-0711-6335 10.61186/jsmt.21.26.118 The researchers conducted the present study with the aim of investigating the pain and muscle disorders of patients with covid-19 with people who have experienced it. The research was applied and comparative. Samples were calculated using G-power of 303 people, and sports teachers were excluded from the research. The Nordic questionnaire was provided to Ardabil education and training employees after the investigation, the number of people with one year of identification and the number of 113 people with the age range of 30 to 45 years reported no reduction. Then, musculoskeletal disorders of 9 body areas in both groups were evaluated using descriptive statistics and chi-square test at a significance level of 0.01. The muscle pain of the affected group was significantly (P<0.01) higher than that of the healthy group, and also based on the chi-square test, the amount of back and neck pain was 52% and 45%, respectively, in affected people compared to other organs. It was more meaningful. (P<0.01) noticed that there was no significant difference between the pain and discomfort of different organs in the non-observed group. Corona patients may experience pain, and the cause of it in the Nordic questionnaire, in addition to physical abnormalities and excessive muscle disease, can be from Corona disease; Therefore, the Nordic questionnaire should be used. Pain, COVID19, Musculoskeletal Disorders. http://jsmt.khu.ac.ir/article-1-529-en.html http://jsmt.khu.ac.ir/article-1-529-en.pdf

Kharazmi University Research in Sport Medicine and Technology 2252-0708 2588-3925 21 26 2023 12 1 The Effect Of Combined Training On Aortic Atherosclerosis And FGF23 In Male Rats With Chronic Renal Failure 129 140 FA vahid fazeli Kharazmi University vahid7fazeli@yahoo.com N 0000-0002-7933-7014 Pezhman Motamedi Kharazmi Of University pezhman.motamedi@khu.ac.ir Y 0000-0001-6092-9098 Hamid Rajabi Kharazmi Of University N 0000-0002-3276-1598 Neda Khaledi Kharazmi Of University N 0000-0002-3859-513X 10.61186/jsmt.21.26.129 Objective: The aim of the present study was to investigate the effect of combined exercise on aortic atherosclerosis and FGF23 in male rats with chronic renal failure. Methods: In this study, 20 male Wistar rats (mean weight 10 ± 250  g) were induced by NX5 / 6 method for chronic renal failure. After 2 weeks of recovery, they were randomly divided into two groups of control (n= 10) and combined training (n= 10) and performed a combined training protocol for 8 weeks. The combined training protocol consisted of two resistance training exercises on a ladder with 2 sets and 6 repetitions with percentages of body weight and aerobic exercise included 30 minutes of low-intensity treadmill activity. Results: The results showed that combined exercise reduced aortic atherosclerosis and decreased plaques, and FGF23 decreased significantly (p = 0.001). Parathoromon hormone also significantly decreased (p = 0.041) and calcium and phosphorus significantly decreased (p = 0.004 and p = 0.005, respectively). Conclusion: Overall, the results showed that combined training could decrease FGF23, parathoromon hormone, calcium and phosphor on aortic atherosclerosis and cardiovascular disease indices in chronic renal failure patients. Exercise, Minerals, Dialysis, Glomerulus, Left Ventricle. http://jsmt.khu.ac.ir/article-1-369-en.html http://jsmt.khu.ac.ir/article-1-369-en.pdf

Kharazmi University Research in Sport Medicine and Technology 2252-0708 2588-3925 21 26 2023 12 1 Comparison Of The Effect Of 8-Week Of Neuromuscular Exercises With And Without Cognitive-Dual Tasks On Proprioception And Balance Of Athletes With Anterior Cruciate Ligament Deficiency 141 158 FA Masoud Barzegar Kharazmi Of University msdbarzegar@gmail.com Y 0000-0002-4976-4808 Malihe Hadadnezhad Kharazmi university M.hadadnezhad@yahoo.com N 0000-0002-5826-1524 Sadredin Shojaedin Kharazmi Of university Sa_shojaedin@yahoo.com N 0000-0002-9428-685X Amir Letafatkar Kharazmi Of university Letafatkar.amir@yahoo.com N 0000-0002-5612-8340 rose fouladi Mazandaran Of university N 0000-0002-9796-0774 10.61186/jsmt.21.26.141 The aim of this research was effect of eight weeks of neuromuscular training (NMT) with and without cognitive-dual tasks (DTs) on proprioception and balance of athletes with ACL deficiency (ACL-D). 39 soccer players with ACL-D were selected purposefully and randomly divided into three groups of 13 (control group, neuromuscular training group and neuromuscular training with dual tasks group). Two training groups performed the exercises for eight weeks and three sessions per week. cognitive-motor dual tasks were performed in one of the groups along with neuromuscular exercises in the form of counting down numbers, memorizing numbers, calculating unexpected equations, specialized football skill movements.  The results of the current study indicated that there was no significant difference in knee joint proprioception in the open and closed kinetic chain after applying the intervention between the three groups, but there was a significant difference in lower extremity performance of the athletes based on the Y balance test in the anterior and posterior-lateral direction between the three groups (p<0.05). It seems that providing neuromuscular exercises along with cognitive-dual tasks can probably be considered as a useful approach to improve lower extremity performance based on the Y balance test in athletes with anterior cruciate ligament deficiency.   Soccer, Anterior Cruciate Ligament Deficiency, Neuromuscular Training, Dual Tasks. http://jsmt.khu.ac.ir/article-1-591-en.html http://jsmt.khu.ac.ir/article-1-591-en.pdf

Kharazmi University Research in Sport Medicine and Technology 2252-0708 2588-3925 21 26 2023 12 1 The Effect Of Different Doses Of Caffeine Chewing Gum On Stress Hormones And Shooting Performance Of Military Men 159 171 FA hamdollah hadi Assistant Professor Of Police University amir.hadi1@gmail.com Y 0000-0002-5432-8031 10.61186/jsmt.21.26.159 The purpose of this research was to determine the effect of different doses of caffeine chewing gum on stress hormones and shooting performance of military men. The subjects of the present research were 10 commanders of Valiasr Police Training Center in Tabriz with a mean and standard deviation of age of 31.52±2.78 years, weight of 75.32±3.91 kilograms, His height was 176.14 ± 4.84 cm. The levels of cortisol, adrenocorticotropic hormone and oxytocin in the blood as well as shooting performance were measured after consuming different doses of caffeine. In the first phase, the placebo was given 15 minutes before the shooting. In the second, third and fourth stages (the time interval between each stage is 5 days), all the subjects were given caffeine gum with doses of 150, 200 and 300 mg 15 minutes before the shooting. Gum and placebo were given to the participants in a double-blind manner. Descriptive statistics, analysis of variance with repeated measures and Tukey's post hoc test were used to analyze the data(P≤0.05). The results of the present study showed that the consumption of caffeine gum in doses of 200 and 300 grams causes a significant decrease in all three stress hormones and also a significant increase in shooting performance, however, the consumption of a dose of 150 mg of caffeine don’t have an effect on the reduction of stress hormones and Shooting performance. According to these results, shooting athletes can be advised to use caffeine gum with doses of 200 and 300 mg before their training and competitions. However, more studies are needed to comment on the use of different doses of caffeine, especially in the form of chewing gum.   Gum, Caffeine, Shooting, Cortisol, Stress http://jsmt.khu.ac.ir/article-1-629-en.html http://jsmt.khu.ac.ir/article-1-629-en.pdf