, Jafar Mohamadian,
Volume 10, Issue 4 (2-2011)
Abstract
Halophilic bacteria can not growth without NaCl and they have optimum growth in high salinity media. Halobacteria are chemoorganotroph and the most species are aerobic. Bacteriorhodopsin is a light driven proton pump that found only in halobacteria. In this study H. salinarum that isolated in previous research from saline environment in Iran were used for isolation of purple membrane. The method of Yucel et al (1995) was applied for isolation of purple membrane of H. salinarum. The stages of this method include dialysis and centrifuge at high speed. Spectrophotometery analysis at 560 nm and absorbance ratio 280/560 nm were used for conform the purity of purple membrane. The activity of purple membrane was assayed as change of pH in salt solution contains MgCl2 and KCl. By using of microwave radiation apparatus the ability of microwave absorbance were measured. The result of isolation of purple membrane indicated that the 280/560 absorbance ratio in this spectrum was 3/3 that confirm the purity of purple membrane. Extracted purple membrane from 4 liter of culture media was 35 milligram. This extracted PM could decrease of pH (0.15 units per 6 hours) and absorb 60 % of microwave radiation. In conclusion there is very diversity between halophilic archaea in Iran ecosystem and extracted PM is favorable for nanotechnology application
Volume 18, Issue 44 (10-2009)
Abstract
The increasing use of microwave appliances such as mobile phones in daily life has caused some anxiety about its possible effects on human health. In this study, we investigated the effects of microwave on the gonads and fertility rate of female Balb /C mouse. At first, an experimental generator microwave system was designed. Then, adult virgin females were exposed to waves for 4 days (6 hours per day) and their blood were applied for hormone assay of FSH, LH, estradiol, progesterone. The size and weight of ovaries were recorded and the structure and ultrastructure of them were examined by light and transmission electron microscope. Some females were placed in a cage with male individual of the same strain for mating, then the pregnancy rate was estimated and their offsprings were maintained in animal house until puberty. After puberty, ovaries were examined. The results did not show any significant difference in the size and weight of ovaries (P> 0.05), FSH, LH, estradiol, did not show change (P>0.05), but the results of transmission electron microscopy showed changes in ovary and follicle structure and also progesterone levels. The percentage of mating showed a significant reduction (P<0.05).
