Volume 8, Issue 4 (11-2010)
Abstract
Salvia leriifolia (Lamiaceae) is an endemic plant of Khorasan and Semnan provinces which has antinociceptive, anti-inflammatory, antidiabetic and antioxidant properties. In this research, antibacterial activity of of S. leriifolia leaf was investigated at different growth stages and the best harvesting time was determined. The leaves were harvested at vegetative (mid March), flowering (mid April) and ripening seed stages (late May). Different concentrations of metanolic extract(5,10,15 and 20 g/l) of leaves were applied on five different bacteria(Pseudomonas aeruginosa, Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli and Proteus mirabilis) according to dick diffusion method. Inhibition zones were measured after 24 hours. Inhibitory effect of extracts at different stages were compared together and with certain antibiotics (Penicillin, Ampicilin and Tetracaiclin). Statistical analysis was performed through the JMP software. Maximum antibacterial activity of leaves was coincident with flowering stage. In this stage, antibacterial activity of leaves was significantly higher than the vegetative and ripening seed stage. Inhibitory effect of leaf extract at flowering stage on some bacteria was higher than the antibiotics or similar with them. It seems that flowering stage (mid April) is the best time for obtaining the maximum antioxidant activity of leaf.
Parvaneh Abrishamchi, Samane Attaran Dowom, ,
Volume 15, Issue 2 (2-2015)
Abstract
Free radicals especially various kind of active oxygens, with destruction of biomolecules such as DNA and proteins, are believed to be the cause of many cancers. In search for antioxidative reagents, it seems that use of natural antioxidants especially those from plants, are of special interest. Salvia leriifolia Benth, from Lamiaceae (syn. Labiatae) family, is a native plant of Iran ( North of Khorasan) and Afghanestan with significant applications in medicine, pharmacology, and food industry. The aim of current investigation, was to recognize the composition of essential oil of S. leriifolia and perform a comparative study on the antioxidant properties of the extracted essence from plants grown in two regions of Khorasan (Neyshabour and Bajestan). According to Gas Chromatography – Mass spectrophotometric analysis, 1,8 Cineole, &alpha-Pinene and &beta-Pinene, were main components of the essential oilAntioxidant activity of essential oil were measured by three different methods, TBARS and BCB (two lipid systems) and DPPH (aqueous system). Antioxidative properties of the essence in both former methods were higher than DPPH assay. It was found that the total essential oil and its pure constituents have a significant antioxidant effect when tested by each method, respectively. BCB assay was the most appropriate method for measurement of antioxidant activity. The antioxidant concentrations influenced its antioxidant power, too. The essence of plants collected from Neyshabour showed stronger antioxidative effect compared to those from Bajestan.
