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Volume 10, Issue 3 (2-2010)
Abstract
Acute Promyelocytic Leukemia (APL) is a kind of acute Leukemia. L-Ascorbic Acid (L-AA) also known as Vitamin C has anti-oxidant properties. L-AA under certain conditions acts as a pro-oxidant and it has cytotoxic effect in high dose on different cell lines that this effect depends on its oxidation-reduction properties. Different experiments showed that anti-proliferation and anti- inflammation materials could influence the effect of such components and so decrease their resulting side effects. Regarding to anti-proliferative and anti-cancer Honey Bee Venom (BV), in this research, we examined the effect of BV on L-AA effect of function. The toxic and non-toxic concentrations of L-AA and BV on HL-60 cells were determined and then the effect of these two components individually and also in combination with each other on growth of HL-60 cells were assessed using Trypan blue stained cell counting and MTT assay. All experiments were done three times and data were analyzed using one-way ANOVA test and Instate 3 soft ware. Our finding showed that both BV and L-AA caused cell death at high concentrations in a dose and time dependent manner in HL- these results, it may be suggested that non- toxic concentration of BV can increase anti-60 cells and they could inhibit proliferation of these cells at lower concentrations. L-AA in 0.1 mM concentration inhibited proliferation of HL-60 cells during 72 h and this inhibitory effect of L-AA significantly increased in combination with BV. On the basis of proliferation potency of L-AA on HL-60 cancer cell line.
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Volume 12, Issue 3 (11-2012)
Abstract
Umbilical cord (UC) is an important source of multipotential mesenchymal stem cells (MSCs). MSCs can be differentiated into different types of cells if they cultured under specific conditions. It has been proved that vitamin D3 can cause differentiation of stem cells in to osteoblast. Also, it has been observed that bee venom (BV) is effective in differentiation of cancerous cells. In this study differentiating potential of BV and vitamin D3 on MSCs to osteoblast was examined. Furthermore, our hypothesis was that BV could increase differentiating potential of vitamin D3. The cells obtained from UC tissues of 10-12 mouse embryos which were digested enzymatically and suspended in DMEM medium. For approving of stem cells, embryonic Oct4 marker was checked and the mesenchymal character of these cells was proven by surface markers including CD73, CD29, CD44. The flowcytometric analysis revealed high levels of these markers. After the second passage, in order to induce osteogenic differentiation, cells were cultured for 21days in DMEM medium containing different concentrations of BV, vitamin D3 separately and BV/vitamin D3 together. At first, cytotoxic effects of BV on MSCs were tested by MTT assay, which were shown that BV inhibited MSCs growth at higher concentrations than 6&mug/ml. Following the treatment, calcium’s level in the cells was determined by Alizarin red staining. Also, as an osteogenic marker, alkalin phosphatase level was measured in treated and non-treated cells. By Alizarin red staining and alkalin phosphatase assay, we found that BV With non-toxic concentrations (2µgr/ml, 4µgr/ml and 6µgr/ml) can cause a few osteogenic differentiation of MSCs. Whereas, usage of BV/vitamin D3 together, caused increasing the differential effects of vitamin D3. In conclusion we suggest that if MSCs were treated with bee venom and vitamin 3 at the same time, these components able to differentiate MSCs to osteoblast, therefore, they could be useful in cell therapy.
