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Acute Promyelocytic Leukemia (APL) is a kind of acute Leukemia. L-Ascorbic Acid (L-AA) also known as Vitamin C has anti-oxidant properties. L-AA under certain conditions acts as a pro-oxidant and it has cytotoxic effect in high dose on different cell lines that this effect depends on its oxidation-reduction properties. Different experiments showed that anti-proliferation and anti- inflammation materials could influence the effect of such components and so decrease their resulting side effects. Regarding to anti-proliferative and anti-cancer Honey Bee Venom (BV), in this research, we examined the effect of BV on L-AA effect of function. The toxic and non-toxic concentrations of L-AA and BV on HL-60 cells were determined and then the effect of these two components individually and also in combination with each other on growth of HL-60 cells were assessed using Trypan blue stained cell counting and MTT assay. All experiments were done three times and data were analyzed using one-way ANOVA test and Instate 3 soft ware. Our finding showed that both BV and L-AA caused cell death at high concentrations in a dose and time dependent manner in HL- these results, it may be suggested that non- toxic concentration of BV can increase anti-60 cells and they could inhibit proliferation of these cells at lower concentrations. L-AA in 0.1 mM concentration inhibited proliferation of HL-60 cells during 72 h and this inhibitory effect of L-AA significantly increased in combination with BV. On the basis of proliferation potency of L-AA on HL-60 cancer cell line.

Keywords: L-Ascorbic Acid, Honey Bee Venom, HL-60 Acute Promyelocytic Leukemia cell line, anti- proliferation

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