Volume 9, Issue 4 (__1334385454.pdf 2010)
2010, 9(4): 603-612 |
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The study of antimutation and anticarcinogen activity of triterpenoids presented in apple peel( Ursolic Acid and oleanolic acid) by the use of Ames test.. Journal title 2010; 9 (4) :603-612
URL: http://jsci.khu.ac.ir/article-1-1447-en.html
URL: http://jsci.khu.ac.ir/article-1-1447-en.html
Abstract: (8988 Views)
The Ursolic Acid and Oleanolic Acid presented in apple peels have a potential antioxidant acivity. Antiprolifertive acivity of the triterpenoids against human liver cancer cells, breast cancer cells and colon cancer ells is evaluated. In this study, we have used Ursolic Acid and Oleanolic Acid which are predominant isolated triterpenoids of the apple peels.. The purpose of this study is to determine antimutation and anticarcinogen effect of Ursolic Acid and Oleanolic Acid against Sodium Azide mutagen substance by the use of Microsome and Ames test.
These substances were white powder and soluble in Acetone and bought from sigma company. The bacterium used in this test is a strain of Salmonella thyphimurium (TA100) that carries a selective mutation in its Histidine Operon. We also added Microsome to improve anticancer activity of this two triterpenoids. In this study, inhibition percentage from mutation was 83% for ursolic acid and 76.4 % for oleanolic acid in presence of Microsome ( and 77.6 % for Ursolic Acid and 69.8 % for Oleanolic Acid in absence of Microsome) . Therefore , presence of anti mutation Ursolic Acid and oleanolic acid along with mutagen substance, reduced quantity of back mutation and Inhibition over 40 % recommended as a powerful antimutation substance. Therefore, apple peel has large amount of active Phytochemicals, with Antimutation and Anticarcinogen activity and Ursolic Acid has more antimutation activity than Oleanolic acid
These substances were white powder and soluble in Acetone and bought from sigma company. The bacterium used in this test is a strain of Salmonella thyphimurium (TA100) that carries a selective mutation in its Histidine Operon. We also added Microsome to improve anticancer activity of this two triterpenoids. In this study, inhibition percentage from mutation was 83% for ursolic acid and 76.4 % for oleanolic acid in presence of Microsome ( and 77.6 % for Ursolic Acid and 69.8 % for Oleanolic Acid in absence of Microsome) . Therefore , presence of anti mutation Ursolic Acid and oleanolic acid along with mutagen substance, reduced quantity of back mutation and Inhibition over 40 % recommended as a powerful antimutation substance. Therefore, apple peel has large amount of active Phytochemicals, with Antimutation and Anticarcinogen activity and Ursolic Acid has more antimutation activity than Oleanolic acid
Keywords: Apple peel, Antimutation and anticancer activity, oleanolic acid, Ursolic Acid, Sallmonella thyphimurium
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