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In this research the effects of insulin and insulin +hCG on the sexual activity of male gonad of Bufo viridis have been investigated. The familiar manifestations of sexual activity in amphibian, such as clasping, mating call, associated with histological changes have been studied. The object of this study was to examine reproductive cycle under experimental condition. The experimental animals consisted of four groups in each we used 5 mature male toads ranging 23-25gr of body weight. Animals of first experimental group received insulin 4 times during a 2 week period with every 3 day interval intraperitoneally (IP). The first injection was 7 IU/kg B.W. and the others were 3.5 IU/kg B.W. The second experimental group received insulin together with hCG 4 times during a 2 week period with every 3 day intervals intraperitoneally (IP). The first injection was 7 IU/kg B.W insulin and 50 IU/ml hCG and the others were 3.5 IU/kg B.W insulin with 50 IU/ml hCG. The control groups received Ringer solution in each injection. The researchers found that in the second experimental group after third injection skin became darker and sexual behavior such as mating calls and clasping were appeared. The testes of experimental animals were histologically studied. The number and diameter of seminiferous tubules and number of spermatogenic cells in first and second experimental groups were increased. The researchers can conclude that insulin caused cell division and increased number of spermatogenic cells but did not affect on sexual behavior, where as hCG produced sexual activities

Keywords: Insulin, Insulin and hCG, Testis, Spermatogenesis, Bufo

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